Molecular Cloning and Characterization of a Novel Cold-Adapted Family VIII Esterase from a Biogas Slurry Metagenomic Library
文献类型： 外文期刊
作者： Cheng, Xiaojie 1 ; Wang, Xuming 1 ; Qiu, Tianlei 1 ; Yuan, Mei 2 ; Sun, Jianguang 2 ; Gao, Junlian 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Municipal Key Lab Agr Gene Resources & Bi, Beijing 100097, Peoples R China
2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources, Beijing 100081, Peoples R China
关键词： Lipolytic enzyme;family VIII esterase;cold-adapted esterase;metagenomic library;biogas digester
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at 10 degrees C (43% activity remained), with the optimal temperature at 20 degrees C, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.
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