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Deletion of the znuA virulence factor attenuates Actinobacillus pleuropneumoniae and confers protection against homologous or heterologous strain challenge

文献类型： 外文期刊

作者： Yuan, Fangyan 1 ; Liao, Yonghong 3 ; You, Wujin 2 ; Liu, Zewen 1 ; Tan, Yongqiang 2 ; Zheng, Chengkun 2 ; BinWang 2 ; Zho 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, State Key Lab Agr Microbiol, Div Anim Infect Dis, Wuhan 430070, Peoples R China

3.Luoyang Pulike Bioengn Co Ltd, Natl Res Ctr Vet

关键词： Actinobacillus pleuropneumoniae;znuA gene;Deletion mutant;Virulence;Protection

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： The znuA gene is known to be important for growth and survival in Escherichia coli, Haemophilus spp., Neisseria gonorrhoeae, and Pasteurella multocida under low Zn2+ conditions. This gene is also present in Actinobacillus pleuropneumoniae serotype 1; therefore, the aim of this study was to investigate the existence of a similar role for the znuA gene in the growth and virulence of this organism. A precisely defined Delta znuA deletion mutant of A. pleuropneumoniae was constructed based on the sequence of the wild-type SLW01 using transconjugation and counterselection. This mutation was found to be lethal in low-Zn2+ medium. Furthermore, the Delta znuA mutant strain exhibited attenuated virulence (>= 22-fold) as well as reduced mortality and morbidity in a murine (Balb/C) model of infection. The majority of the bacteria were cleared from the lungs within 2 weeks. The Delta znuA mutant strain caused no adverse effects in pigs at doses of up to 1.0 x 10(9) CFU/mL. The Delta znuA mutant strain induced a significant immune response and conferred 80% and 100% protection on immunised pigs against challenge with A. pleuropneumoniae strains belonging to homologous or heterologous serovars, respectively, compared to the blank controls. The data obtained in this study indicate the potential of the mutant Delta znuA strain for development as a live vaccine capable of inducing reliable cross-serovar protection following intratracheal immunisation. (C) 2014 Elsevier B.V. All rights reserved.

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