Tobacco Arabinogalactan Protein NtEPc Can Promote Banana (Musa AAA) Somatic Embryogenesis
文献类型： 外文期刊
作者： Shu, H. 1 ; Xu, L. 4 ; Li, Z. 4 ; Li, J. 1 ; Jin, Z. 1 ; Chang, S. 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou, Peoples R China
2.Key Iab Hainan Banana Genet & Breeding, Haikou, Peoples R China
3.Zhengzhou Univ, Dept Biol, Zhengzhou 450052, Peoples R China
4.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Danzhou, Peoples R China
关键词： Arabinogalactan protein;NtEPc;Banana (Musa AAA);Somatic embryogenesis
期刊名称：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：2.926； 五年影响因子：2.685 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Banana is an important tropical fruit worldwide. Parthenocarpy and female sterility made it impossible to improve banana varieties through common hybridization. Genetic transformation for banana improvement is imperative. But the low rate that banana embryogenic callus was induced made the transformation cannot be performed in many laboratories. Finding ways to promote banana somatic embryogenesis is critical for banana genetic transformation. After tobacco arabinogalactan protein gene NtEPc was transformed into Escherichia coli (DE3), the recombinant protein was purified and filter-sterilized. A series of the sterilized protein was added into tissue culture medium. It was found that the number of banana immature male flowers developing embryogenic calli increased significantly in the presence of NtEPc protein compared with the effect of the control medium. Among the treatments, explants cultured on medium containing 10 mg/l of NtEPc protein had the highest chance to develop embryogenic calli. The percentage of lines that developed embryogenic calli on this medium was about 12.5 %. These demonstrated that NtEPc protein can be used to promote banana embryogenesis. This is the first paper that reported that foreign arabinogalactan protein (AGP) could be used to improve banana somatic embryogenesis.
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