文献类型： 外文期刊
作者： Wu, Hong-xia 1 ; Jia, Hui-min 1 ; Ma, Xiao-wei 2 ; Wang, Song-biao 2 ; Yao, Quan-sheng 2 ; Xu, Wen-tian 3 ; Zhou, Yi-g 1 ;
作者机构： 1.Zhejiang Univ, State Agr Minist, Lab Hort Plant Growth Dev & Qual Improvement, Dept Hort, Hangzhou 310058, Zhejiang, Peoples R China
2.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minist Agr, Key Lab Trop Fruit Biol, Zhanjiang 524091, Guangdong, Peoples R China
3.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minis
期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：4.044； 五年影响因子：4.02 ）
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收录情况： SCI
摘要： Here we used Illumina RNA-seq technology for transcriptome sequencing of a mixed fruit sample from 'Zill' mango (Mangifera indica Linn) fruit pericarp and pulp during the development and ripening stages. RNA-seq generated 68,419,722 sequence reads that were assembled into 54,207 transcripts with a mean length of 858 bp, including 26,413 clusters and 27,794 singletons. A total of 42,515(78.43%) transcripts were annotated using public protein databases, with a cut-off E-value above 10~(-5), of which 35,198 and 14,619 transcripts were assigned to gene ontology terms and clusters of orthologous groups respectively. Functional annotation against the Kyoto Encyclopedia of Genes and Genomes database identified 23,741(43.79%) transcripts which were mapped to 128 pathways. These pathways revealed many previously unknown transcripts. We also applied mass spectrometry-based transcriptome data to characterize the proteome of ripe fruit. LC-MS/MS analysis of the mango fruit proteome was using tandem mass spectrometry (MS/MS) in an LTQ. Orbitrap Velos (Thermo) coupled online to the HPLC. This approach enabled the identification of 7536 peptides that matched 2754 proteins. Our study provides a comprehensive sequence for a systemic view of transcriptome during mango fruit development and the most comprehensive fruit proteome to date, which are useful for further genomics research and proteomic studies.
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