Identification of the passion fruit (Passiflora edulis Sims) MYB family in fruit development and abiotic stress, and functional analysis of PeMYB87 in abiotic stresses
文献类型： 外文期刊
作者： Zhang, Yan-shu 1 ; Xu, Yi 1 ; Xing, Wen-ting 1 ; Wu, Bin 1 ; Huang, Dong-mei 1 ; Ma, Fu-ning 1 ; Zhan, Ru-lin 1 ; Sun, Pei-guang 1 ; Xu, Yong-yan 4 ; Song, Shun 1 ;
作者机构： 1.Trop Crops Genet Resources Inst, CATAS Germplasm Repository Passiflora, Natl Key Lab Trop Crop Breeding, Haikou Expt Stn, Haikou, Hainan, Peoples R China
2.Chinese Acad Trop Agr Sci, Sanya Res Inst, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Sanya, Hainan, Peoples R China
3.Hainan Yazhou Bay Seed Lab, Sanya, Hainan, Peoples R China
4.Southwest Forestry Univ, Coll Landscape & Hort, Kunming, Yunnan, Peoples R China
关键词： passion fruit; MYB; PeMYB87; abiotic stress; expression analysis
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Environmental stresses are ubiquitous in agricultural cultivation, and they affect the healthy growth and development of edible tissues in passion fruit. The study of resistance mechanisms is important in understanding the adaptation and resistance of plants to environmental stresses. In this work, two differently resistant passion fruit varieties were selected, using the expression characteristics of the transcription factor MYB, to explore the resistance mechanism of the MYB gene under various environmental stresses. A total of 174 MYB family members were identified using high-quality passion fruit genomes: 98 2R-MYB, 5 3R-MYB, and 71 1R-MYB (MYB-relate). Their family information was systematically analyzed, including subcellular localization, physicochemical properties, phylogeny at the genomic level, promoter function, encoded proteins, and reciprocal regulation. In this study, bioinformatics and transcriptome sequencing were used to identify members of the PeMYB genes in passion fruit whole-genome data, and biological techniques, such as qPCR, gene clone, and transient transformation of yeast, were used to determine the function of the passion fruit MYB genes in abiotic stress tolerance. Transcriptomic data were obtained for differential expression characteristics of two resistant and susceptible varieties, three expression patterns during pulp development, and four induced expression patterns under abiotic stress conditions. We further focused on the resistance mechanism of PeMYB87 in environmental stress, and we selected 10 representative PeMYB genes for quantitative expression verification. Most of the genes were differentially induced by four abiotic stresses, among which PeMYB87 responded significantly to high-temperature-induced expression and overexpression of the PeMYB87 gene in the yeast system. The transgenic PeMYB87 in yeast showed different degrees of stress resistance under exposure to cold, high temperatures, drought, and salt stresses. These findings lay the foundation for further analysis of the biological functions of PeMYBs involved in stress resistance in passion fruit.
- 相关文献
作者其他论文 更多>>
-
WRKY transcription factors in passion fruit analysis reveals key PeWRKYs involved in abiotic stress and flavonoid biosynthesis
作者：Ma, Funing;Huang, Dongmei;Xing, Wenting;Wu, Bin;Li, Hongli;Hu, Wenbin;Song, Shun;Xu, Yi;Ma, Funing;Huang, Dongmei;Xing, Wenting;Wu, Bin;Li, Hongli;Hu, Wenbin;Song, Shun;Xu, Yi;Zhou, Hongwu;Yang, Huiting
关键词：Bioinformatics; Transcriptome; Metabolome
-
Passion fruit HD-ZIP genes: Characterization, expression variance, and overexpression PeHB31 enhanced drought tolerance via lignin pathway
作者：Ma, Funing;Song, Shun;Huang, Dongmei;Wu, Bin;Xing, Wenting;Huang, Haijie;Tan, Yuxin;Xu, Yi;Ma, Funing;Song, Shun;Huang, Dongmei;Wu, Bin;Xing, Wenting;Huang, Haijie;Tan, Yuxin;Xu, Yi;Li, Chuanlin;Song, Shun;Xu, Yi
关键词：Transcription factor; Passiflora edulis; Abiotic stress
-
Spatiotemporal dynamic of rice production and its carbon footprint in Hainan, China: Implications for food security and environmental sustainability
作者：Wen, Shu;Qi, Zhiqiang;Wen, Shu;Tian, Butao;Xu, Ling;Liu, Qi;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Wen, Shu;Yao, Wei;Tian, Butao;Xu, Ling;Liu, Qi;Xu, Yi;Yang, Yadong;Zeng, Zhaohai;Zang, Huadong;Qi, Zhiqiang
关键词：
-
Identification of DREB Family Genes in Banana and Their Function under Drought and Cold Stress
作者：Xu, Yi;Zhang, Yanshu;Zhang, Shaoling;Xu, Yi;Ma, Funing;Zhao, Jingxi;Yang, Huiting;Song, Shun;Xu, Yi;Ma, Funing;Zhao, Jingxi;Yang, Huiting;Song, Shun;Xu, Yi;Ma, Funing;Zhao, Jingxi;Yang, Huiting;Song, Shun;Xu, Yi;Ma, Funing;Song, Shun
关键词：genome-wide; DREB; climate change; drought; cold
-
Characterization of the Passion Fruit (Passiflora edulis Sim) bHLH Family in Fruit Development and Abiotic Stress and Functional Analysis of PebHLH56 in Cold Stress
作者：Xu, Yi;Ma, Funing;Huang, Dongmei;Xing, Wenting;Wu, Bin;Sun, Peiguang;Chen, Di;Xu, Binqiang;Song, Shun;Xu, Yi;Ma, Funing;Song, Shun;Zhou, Weidong
关键词：bHLH; passion fruit; abiotic stress; cold; genome-wide analysis
-
Characterization of Dof Transcription Factors and the Heat-Tolerant Function of PeDof-11 in Passion Fruit (Passiflora edulis)
作者：Chen, Ge;Gui, Jie;Huang, Yongcai;Qiu, Wenwu;Yang, Liu;Song, Shun;Xu, Yi;Ma, Funing;Wu, Wenhua;Han, Te;Song, Shun;Xu, Yi;Wu, Wenhua;Han, Te;Xu, Yi;Ma, Funing;Song, Shun
关键词：Dof transcription factor; passion fruit; high-temperature stress; gene expression; transgenic
-
Characterization of the NAC Transcription Factor in Passion Fruit (Passiflora edulis) and Functional Identification of PeNAC-19 in Cold Stress
作者：Xu, Yi;Ma, Funing;Huang, Dongmei;Xing, Wenting;Wu, Bin;Sun, Peiguang;Xu, Binqiang;Song, Shun;Xu, Yi;Ma, Funing;Song, Shun;Xu, Yi;Ma, Funing;Song, Shun;Li, Pengfei
关键词：NAC transcription factor; passion fruit; abiotic stress; fruit maturity stage; gene expression
