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Artificial leaf aids analysis of chlorophyll fluorescence and P700 absorbance in studies involving microalgae

文献类型： 外文期刊

作者： Qiao, Hongjin 1 ; Fan, Xiao 2 ; Xu, Dong 2 ; Ye, Naihao 2 ; Wang, Jiying 1 ; Cao, Shaona 3 ;

作者机构： 1.Shandong Marine Resource & Environm Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

3.Qingdao Agr Univ, Qingdao, Peoples R China

关键词： artificial leaf;cuvette;filter membrane;microalgae;prediction analysis of microarrays

期刊名称：PHYCOLOGICAL RESEARCH （ 影响因子：1.675； 五年影响因子：1.87 ）

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收录情况： SCI

摘要： Measuring chlorophyll fluorescence and P700 absorbance has been widely used to study photosynthesis in both terrestrial plants and algae. However, in order to apply these measurement techniques to study microalgae, a concentrated suspension of algae, which is usually prepared by centrifugation, is required. In this study, instead of using centrifugation, we concentrated microalgae on a nitrocellulose membrane using filtration to create an artificial leaf' before analysis. Overall, we were able to generate values of the appropriate photosynthetic parameters that were comparable to those obtained when chlorophyll fluorescence and P700 absorbance were measured following centrifugation. There were no statistically significant differences (P>0.05) between the artificial leaf method and the traditional cuvette method for determining chlorophyll fluorescence or P700 absorbance at appropriate chlorophyll concentrations. We were also able to reduce background noise by using a filter membrane as a carrier. Therefore, an artificial leaf has the potential to be a valuable tool for phycologists interested in studying microalgal photosynthesis by enabling them to eliminate tedious centrifugation steps. In addition, fluorometers commonly used for studying the leaves of higher plants will also be suitable for studying microalgae.

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