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Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (Paralichthys olivaceus)

文献类型： 外文期刊

作者： Li, Jie 1 ; Mo, Zhaolan 1 ; Li, Guiyang 1 ; Xiao, Peng 3 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

2.Natl Lab Marine Sci & Technol, Qingdao, Peoples R China

3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China

关键词： Attenuated mutant;Edwardsiella tarda;Edwardsiellosis;Immune protection;Paralichthys olivaceus

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

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收录情况： SCI

摘要： Edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. The development of a live attenuated vaccine may be an effective approach for preventing this disease in fish. In this study, we introduced deletions of esrB, esaC, evpH, rpoS, and purA into the E. tarda LSE40 Delta aroA strain, thereby generating five double-gene mutants (6,aroA,6,esrB, Delta aroA Delta esaC, Delta aroA Delta rpoS, Delta aroA Delta evpH, and Delta arolA Delta purA) and two triple-gene mutants (Delta aroA Delta esrB Delta evpH and Delta aroA Delta esaC Delta evpH). When blue gourami (Trichogaster trichopterus) was used as a fish model for the primary screening and evaluation of the vaccine candidates, all mutants were attenuated significantly by more than 2 to 3 logs in terms of the 50% lethal dose (LD50). Five double-gene mutants yielded relative percentage survival (RPS) rates of 26.1 82.6% after challenge with wild-type E. tarda. The Delta aroA Delta esrB mutant that conferred the highest RPS (82.6%) in blue gourami was also evaluated in flounder (Paralichthys olivaceus). After vaccination via intramuscular (i.m.) injection or immersion, this mutant could persist in the flounder for 14-35 days and it induced higher serum antibody titers than the control fish (P < 0.01). Flounder vaccinated via i.m. injection at doses of 10(3)-10(7) CFU/flsh had RPS rates of 14.3-66.7% after i.m. challenge with 104 CFU/fish using wild-type E. tarda. Flounder vaccinated via immersion at a dose of 10(7) CFU/ml exhibited 100% RPS against immersion challenge with 10(7) CFU/ml using wild-type E. tarda. These results indicate that the Delta aroA Delta esrB mutant could be used as an effective live vaccine to combat edwardsiellosis in flounder. (C) 2015 Elsevier Ltd. All rights reserved.

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[1]Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin,Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin.

[2]Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR. Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Sun, Li,Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Wang Fang.

[3]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[4]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[5]Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). Xu, J-Y,Chen, S-L,Ding, H.,Xu, J-Y. 2009

[6]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[7]Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[8]A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection. Sun, Y.,Hu, Y-H.,Sun, L.,Sun, Y.,Liu, C-S.. 2012

[9]Optimization of protectant, salinity and freezing condition for freeze-drying preservation of Edwardsiella tarda. Yu, Yongxiang,Xue, Liangyi,Yu, Yongxiang,Zhang, Zheng,Wang, Yingeng,Liao, Meijie,Li, Bin. 2017

[10]MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families. Du Min,Chen SongLin,Yang JingFeng,Zhang Bo,Du Min,Liu YanHong,Niu BaoZhen,Du Min. 2012

[11]Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[12]Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant. Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,Xiao, Peng. 2013

[13]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[14]Domain analysis of the Edwardsiella tarda ferric uptake regulator. Sun, Kun,Cheng, Shuang,Wang, Fang,Sun, Li,Sun, Kun,Cheng, Shuang,Wang, Fang,Wang, Fang.

[15]Estimation of genetic parameters for growth related traits at different stages of development in Paralichthys olivaceus (Temminck & Schlegel, 1846). Liu, F.,Liu, F.,Chen, S. L.,Wang, L.,Zhang, Y. P.,Tian, Y. S.,Chen, H. L.,Liu, F.,Chen, S. L.,Wang, L.,Zhang, Y. P.,Tian, Y. S.,Chen, H. L.,Wang, L.,Chen, H. L..

[16]Genetic verification of doubled haploid Japanese flounder, Paralichthys olivaceus by genotyping telomeric microsatellite loci. Liu, Yongxin,Liu, Haijin,Wang, Guixing,Wang, Yufen,Si, Fei,Sun, Zhaohui,Zhang, Xiaoyan,Liu, Yi,Hou, Jilun. 2012

[17]Genetic differentiation among common and selected hatchery populations of founder: Evidence from RAPD markers. Liu, Yun-Guo,Chen, Song-Lin,Li, Ba-Fang. 2007

[18]Analysis of genetic variation in selected stocks of hatchery flounder, Paralichthys olivaceus, using AFLP markers. Liu, YG,Chen, SL,Li, BF,Wang, ZJ,Liu, ZJ. 2005

[19]Optimization of the purification methods for recovery of recombinant growth hormone from Paralichthys olivaceus. Liu Bin,Zang Xiaonan,Zhang Xuecheng,Mu Xiaosheng. 2013

[20]Assessing the genetic structure of three Japanese flounder (Paralichthys olivaceus) stocks by microsatellite markers. Liu, YG,Chen, SL,Li, BF. 2005

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