您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder (Paralichthys olivaceus)

文献类型： 外文期刊

作者： Li, Jie 1 ; Mo, Zhaolan 1 ; Li, Guiyang 1 ; Xiao, Peng 3 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

2.Natl Lab Marine Sci & Technol, Qingdao, Peoples R China

3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China

关键词： Attenuated mutant;Edwardsiella tarda;Edwardsiellosis;Immune protection;Paralichthys olivaceus

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. The development of a live attenuated vaccine may be an effective approach for preventing this disease in fish. In this study, we introduced deletions of esrB, esaC, evpH, rpoS, and purA into the E. tarda LSE40 Delta aroA strain, thereby generating five double-gene mutants (6,aroA,6,esrB, Delta aroA Delta esaC, Delta aroA Delta rpoS, Delta aroA Delta evpH, and Delta arolA Delta purA) and two triple-gene mutants (Delta aroA Delta esrB Delta evpH and Delta aroA Delta esaC Delta evpH). When blue gourami (Trichogaster trichopterus) was used as a fish model for the primary screening and evaluation of the vaccine candidates, all mutants were attenuated significantly by more than 2 to 3 logs in terms of the 50% lethal dose (LD50). Five double-gene mutants yielded relative percentage survival (RPS) rates of 26.1 82.6% after challenge with wild-type E. tarda. The Delta aroA Delta esrB mutant that conferred the highest RPS (82.6%) in blue gourami was also evaluated in flounder (Paralichthys olivaceus). After vaccination via intramuscular (i.m.) injection or immersion, this mutant could persist in the flounder for 14-35 days and it induced higher serum antibody titers than the control fish (P < 0.01). Flounder vaccinated via i.m. injection at doses of 10(3)-10(7) CFU/flsh had RPS rates of 14.3-66.7% after i.m. challenge with 104 CFU/fish using wild-type E. tarda. Flounder vaccinated via immersion at a dose of 10(7) CFU/ml exhibited 100% RPS against immersion challenge with 10(7) CFU/ml using wild-type E. tarda. These results indicate that the Delta aroA Delta esrB mutant could be used as an effective live vaccine to combat edwardsiellosis in flounder. (C) 2015 Elsevier Ltd. All rights reserved.

  • 相关文献

[1]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[2]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[3]Specific MHC class II B alleles associated with resistance to Edwardsiella tarda in turbot, Psetta maxima (L.). Xu, J-Y,Chen, S-L,Ding, H.,Xu, J-Y. 2009

[4]A Streptococcus iniae DNA vaccine delivered by a live attenuated Edwardsiella tarda via natural infection induces cross-genus protection. Sun, Y.,Hu, Y-H.,Sun, L.,Sun, Y.,Liu, C-S.. 2012

[5]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[6]Identification of an Edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[7]Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain. Sun, Yun,Liu, Chun-sheng,Sun, Li,Sun, Yun,Liu, Chun-sheng. 2010

[8]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[9]Optimization of protectant, salinity and freezing condition for freeze-drying preservation of Edwardsiella tarda. Yu, Yongxiang,Xue, Liangyi,Yu, Yongxiang,Zhang, Zheng,Wang, Yingeng,Liao, Meijie,Li, Bin. 2017

[10]Phenotypic characterization, virulence, and immunogenicity of Edwardsiella tarda LSE40 aroA mutant. Mo, Zhao-Lan,Li, Jie,Li, Gui-Yang,Xiao, Peng. 2013

[11]MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families. Du Min,Chen SongLin,Yang JingFeng,Zhang Bo,Du Min,Liu YanHong,Niu BaoZhen,Du Min. 2012

[12]Regulation of the Edwardsiella tarda Hemolysin Gene and luxS by EthR. Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Sun, Li,Wang Fang,Zhang, Min,Hu, Yong-hua,Zhang, Wei-wei,Wang Fang.

[13]Domain analysis of the Edwardsiella tarda ferric uptake regulator. Sun, Kun,Cheng, Shuang,Wang, Fang,Sun, Li,Sun, Kun,Cheng, Shuang,Wang, Fang,Wang, Fang.

[14]Proteome profiling reveals immune responses in Japanese flounder (Paralichthys olivaceus) infected with Edwardsiella tarda by iTRAQ analysis. Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin,Wang, Lei,Shao, Changwei,Xu, Wenteng,Zhou, Qian,Wang, Na,Chen, Songlin.

[15]Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Liu, YG,Chen, SL,Li, J,Li, BF. 2006

[16]Molecular identification and expression analysis of natural resistance associated macrophage protein (Nramp) cDNA from Japanese flounder (Paralichthys olivaceus). Chen, SL,Wang, ZJ,Xu, MY,Gui, JF. 2006

[17]MHC polymorphism and disease resistance to Vibrio anguillarum in 12 selective Japanese flounder (Paralichthys olivaceus) families. Xu, Tian-jun,Chen, Song-lin,Ji, Xiang-shan,Tian, Yong-sheng,Xu, Tian-jun. 2008

[18]MHC class II alpha gene polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus). Xu, Tian-jun,Chen, Song-lin,Zhang, Yu-xi,Xu, Tian-jun. 2010

[19]Genetic differentiation among common and selected hatchery populations of founder: Evidence from RAPD markers. Liu, Yun-Guo,Chen, Song-Lin,Li, Ba-Fang. 2007

[20]Phenotypic and genetic parameter estimation of morphological traits related to axial body growth in Japanese flounder. Liu, Yong-Xin,Jiang, Li,Liu, Hai-Jin,Yang, Run-Qing. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台