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Molecular and functional characterization of the JcMYB1, encoding a putative R2R3-MYB transcription factor in Jatropha curcas

文献类型: 外文期刊

作者: Li, Hui-Liang 1 ; Guo, Dong 1 ; Peng, Shi-Qing 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Peoples R China

关键词: Abiotic stress;Gene expression;Jatropha curcas;R2R3 MYB transcription factor;Transgenic tobacco

期刊名称:PLANT GROWTH REGULATION ( 影响因子:3.412; 五年影响因子:3.691 )

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收录情况: SCI

摘要: The cDNA encoding the R2R3MYB transcription factor, designated as JcMYB1, was isolated from Jatropha curcas using rapid amplification of cDNA ends. JcMYB1 contains a 942 bp open reading frame that encodes 313 amino acids. The deduced JcMYB1 protein was predicted to possess the conserved R2R3 domain and the signature motif specific for the interaction between MYB and bHLH proteins in the R3 domain. JcMYB1 is a member of the R2R3-MYB transcription factor subfamily. JcMYB1 expressed at different levels with the highest transcription in the roots, followed by the leaves and stems. JcMYB1 transcription was up-regulated by drought, polyethylene glycol, NaCl and cold treatments. JcMYB1 transcription was up-regulated by abscisic acid and jasmonic acid treatment, whereas JcMYB1 expression was not induced by ethylene. JcMYB1 over-expression improved the drought and salt stress tolerance of transgenic tobacco. JcMYB1 has an important function in modulating responses to abiotic stresses.

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