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Molecular cloning, expression, purification and characterization of vitellogenin in scallop Patinopecten yessoensis with special emphasis on its antibacterial activity

文献类型: 外文期刊

作者: Wu, Biao 1 ; Liu, Zhihong 2 ; Zhou, Liqing 2 ; Ji, Guangdong 1 ; Yang, Aiguo 2 ;

作者机构: 1.Ocean Univ China, Inst Evolut & Marine Biodivers, Lab Evolut & Dev, Dept Marine Biol, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

关键词: Scallop;Patinopecten yessoensis;Vitellogenin;Antibacterial activity;Pattern recognition receptor

期刊名称:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ( 影响因子:3.636; 五年影响因子:3.654 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Vitellogenin (Vg), the major precursor of the egg-yolk proteins, has been found to play an immune role in fish and protochordate amphioxus, however, no study on the immune function of Vg in invertebrates has ever been studied before. In this study, the complete cDNA of Vg was identified from the scallop Patinopecten yessoensis (termed PyVg). The cDNA contained an open reading frame (ORF) of 6888 bp, encoding a polypeptide of 2295 amino acid protein, which had an N-terminal signal peptide followed by the mature Vg. The mature Vg had the domains Vitellogenin_N, domain of unknown function 1943 (DUF1943) and von Willebrand factor type D domain (VWD) as well as the consensus cleavage site (R-X-R/K-R) and conserved motif (KTIGNAG). Tissue distribution assay revealed that PyVg transcripts were predominantly present in the ovary and hepatopancreas, and its expression profile in ovary well reflected the annual cycle of vitellogenesis. Interestingly, bacterial challenge caused a significant change in PyVg expression, hinting an involvement of PyVg in the acute phase response in P. yessoensis. Consistently, recombinant DUF1943 and VWD domains both could interact with LTA and LPS on bacterial wall, and purified native PyVg displayed a broad-spectrum antibacterial activity against both Gram-negative (Escherichia coli and Vibrio anguillarum) and Gram-positive bacteria (Staphylococcus aureus). Overall, these data indicate that Vg is a pattern recognition molecule with bacterial growth-inhibiting activity in the scallop. (C) 2014 Elsevier Ltd. All rights reserved.

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