文献类型： 外文期刊
作者： Wang, Yuan 1 ; Shen, Xiao-Sheng 1 ; Gu, Run Run 1 ; Shi, Yong Fu 1 ; Tian, Liang Liang 1 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fishery Res Inst, Shanghai 200090, Peoples R China
期刊名称：JOURNAL OF FOOD SAFETY （ 影响因子：1.953； 五年影响因子：1.946 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： A double-layer agar plate (DLAP) method was used to detect Vibrio parahaemolyticus in culture broth and seafood samples, which were added with certain amount of V.parahaemolyticus and then subjected to freezing treatment. The results of DLAP, which were compared with the most probable number (MPN) method and direct-plating methods such as Bio-chrome Vibrio medium (BCVM) and thiosulfate-citrate-bile salts-sucrose agar (TCBS), showed that they were not significantly different (P>0.05) from each other under the normal experimental conditions. However, if the bacterium was inactive or injured, the results of DLAP were as effective as MPN and more accurate than those of BCVM and TCBS. Furthermore, DLAP showed the total agreement compared with the MPN method by examining the presence of V.parahaemolyticus in naturally contaminated seafood samples. Because of its sensitivity and exactness, DLAP offers a broad application for fast screening and detecting of V.parahaemolyticus in seafood.
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