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Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum

文献类型： 外文期刊

作者： Liu, Xin 1 ; Xu, Jianhong 1 ; Wang, Jian 1 ; Ji, Fang 1 ; Yin, Xianchao 1 ; Shi, Jianrong 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand Agroprod Safet, Key Lab Food Qual & Safety Jiangsu Prov, State Key Lab Breeding Base,Minist Agr,Inst Food, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Control Technol & Stand

关键词： Threonine dehydratase;Fusarium graminearum;Isoleucine biosynthesis;FgILV1;Virulence

期刊名称：CURRENT GENETICS （ 影响因子：3.886； 五年影响因子：3.697 ）

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收录情况： SCI

摘要： In this study we characterized FgIlv1, a homologue of the Saccharomyces cerevisiae threonine dehydratase (TD) from the important Fusarium head blight fungus Fusarium graminearum. TD catalyzes the first step in the biosynthesis pathway of isoleucine (Ile) for conversion of threonine (Thr) to 2-ketobutyrate (2-KB). The FgILV1 deletion mutant Delta FgIlv1-3 was unable to grow on minimal medium or fructose gelatin agar which lacked Ile. Exogenous supplementation of Ile or 2-KB but not Thr rescued the mycelial growth defect of Delta FgIlv1-3, indicating the involvement of FgIlv1 in the conversion of Thr to 2-KB in Ile biosynthesis. Additionally, exogenous supplementation of Methionine (Met) could also rescue the mycelial growth defect of Delta FgIlv1-3, indicating a crosstalk between Ile biosynthesis and Met catabolism in F. graminearum. Deletion of FgILV1 also caused defects in conidial formation and germination. In addition, Delta FgIlv1-3 displayed decreased virulence on wheat heads and a low level of deoxynivalenol (DON) production in wheat kernels. Taken together, results of this study indicate that FgIlv1 is an essential component in Ile biosynthesis and is required for various cellular processes including mycelial and conidial morphogenesis, DON biosynthesis, and full virulence in F. graminearum. Our data indicate the potential of targeting Ile biosynthesis for anti-FHB management.

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