文献类型： 外文期刊

作者： Song, Wei-Fu ¹ ; Ding, Hai-Yan ² ; Zhang, Xiao-Mei ¹ ; Li, Ji-Lin ¹ ; Xiao, Zhi-Min ³ ; Xin, Wen-Li ³ ; Song, Qing-Jie ³ ;

作者机构： 1.Harbin Normal Univ, Dept Biol, Harbin, Peoples R China

2.Daqing Normal Univ, Sch Life Sci, Daqing, Heilongjiang, Peoples R China

3.Heilongjiang Acad Agr Sci, Crop Breeding Inst, Harbin, Peoples R China

关键词： substitution line hybridization;genomic in situ hybridization;C-banding;wheat variety trials

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

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收录情况： SCI

摘要： Intergeneric crop plant hybrid lines with small-segment chromosome translocations are very useful in plant genetic research and breeding. In this study, to create small-segment chromosome translocations with beneficial agronomic characters, the progeny of wheat-rye substitution lines 5R/5A and 6R/6A were selected from generations F-2 to F-5 for rye-specific characteristics. A PCR primer and specific simple sequence repeat marker for rye were used in F-5 populations to detect rye chromatin and to amplify a specific chromosome band in six translocation lines (06-6-5, 06-6-6, 06-6-9, 6-26-1, 7-23, and 7-33). Fragment pSc119.1 cloned from 7-33 had 99% homology with the big ear gene sequence (GenBank AF512607.1) in wheat. The six lines were further characterized via pollen mother cell meiosis analysis for genetic stability, and chromosome C-banding and genomic in situ hybridization for rye chromatin. The results show that line 7-33 was still within the 5R/5A substitution lines and possessed the big ear gene. The other lines all contained small-segment rye chromosome translocations. The results indicated that substitution line hybridization is an effective method for creating small-segment chromosome translocations with useful agronomic traits. Trials for these six wheat-rye translocation lines are justified because they possess many important stably-inherited agronomic characters, including disease resistance and improved yield.