Novel L-lactic acid biosensors based on conducting polypyrrole-block copolymer nanoparticles
文献类型： 外文期刊
作者： Sun, Chong 1 ; Wang, Daoying 1 ; Zhang, Muhan 1 ; Ni, Yanxiu 2 ; Shen, Xiaohui 3 ; Song, Youchao 3 ; Geng, Zhiming 1 ; Xu 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China
3.Nanjing Normal Univ, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat, Coll Chem & Mat Sci, Nanjing 210023, Peoples R China
期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）
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收录情况： SCI
摘要： The development of advanced nanomaterials for the highly efficient electrical detection of biological species has attracted great attention. Here, novel polypyrrole-Pluronic F127 nanoparticles (PPy-F127 NPs) with conducting and biocompatibility properties were synthesized and used to construct a L-lactic acid biosensor that could be applied in biochemical assays. The PPy-F127 NPs were characterized by transmission electron microscopy (TEM), elemental analysis and UV-vis spectroscopy. Lactate oxidase (LOx) structure variation on the PPy-F127 NPs was investigated by circular dichroism (CD). The cyclic voltammetric results indicated that LOx immobilized on the PPy-F127 NPs exhibited direct electron transfer reaction with a formal potential value (E-0') of 0.154 V vs. SCE. Moreover, the biosensor had good electrocatalytic activity toward L-lactic acid with a wide linear range (0.015-37.5 mM) and a low detection limit of 0.0088 mM. The regression equation was I (mu A) = 0.02353c (mM) + 1.4135 (R-2 = 0.9939). The L-lactic acid biosensor had a good anti-interference property towards uric acid (UA), ascorbic acid (AA), glucose and cysteine. The idea and method provide a promising platform for the rapid development of biosensors that can be used in the detection of biological species.
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