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Development of a GFP-expressing Ustilaginoidea virens strain to study fungal invasion and colonization in rice spikelets

文献类型： 外文期刊

作者： Andargie, Mebeaselassie 1 ; Li, Luoye 1 ; Feng, Aiqing 2 ; Zhu, Xiaoyuan 2 ; Li, Jianxiong 1 ;

作者机构： 1.Chinese Acad Sci, South China Bot Garden, Key Lab South China Agr Plant Mol Anal & Genet Im, Guangzhou 510650, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

关键词： Agrobacterium tumefaciens mediated transformation;EGFP;Insertional mutagenesis;Oryza sativa;Ustilaginoidea virens

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：2.315； 五年影响因子：2.71 ）

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收录情况： SCI

摘要： The fungal pathogen Ustilaginoidea virens is now widespread in most rice producing areas of the world and attacks the seed spikes of rice, causing sterility, reduced seed weight and accumulation of mycotoxins. The lack of appropriate data relating to the mechanisms of infection leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Here, we developed an efficient A. tumefaciens mediated transformation system for rice false smut disease. In addition, infection patterns were explored in rice using the green fluorescent protein gene (gfp) transformed U. virens. ATMT uses both the Hygromycin B resistance (hph) gene and green fluorescent protein as the selection agents. The T-DNA integration into the fungal genome was assessed by PCR. Expression of the gfp gene did not affect the pathogenicity of the U. virens transformants. U. virens pathogenicity was examined on the floral structures using a fluorescence microscope. Fluorescence microscopy allowed in vivo imaging of this pathogen during infection of rice spikelets. These studies will allow us to develop strategies to determine the mechanisms of U. virens-rice interaction in greater detail and to apply functional genomics for the characterization of involved genes at the molecular level either by insertional mutagenesis or gene knock-out. (C) 2014 SAAB. Published by Elsevier B.V. All rights reserved.

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