Co-silence of the coatomer beta and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera
文献类型： 外文期刊
作者： Mao, Jianjun 1 ; Zhang, Peizhuo 2 ; Liu, Changyan 3 ; Zeng, Fanrong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Shanghai GenePharma Co Ltd, Shanghai 201203, Peoples R China
3.Hubei Acad Agr Sci, Food Crops Inst, Wuhan 430064, Peoples R China
关键词： RNAi;siRNA;Helicoverpa armigera
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）
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年卷期：
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收录情况： SCI
摘要： Coatomer and v-ATPase are two genes expressed in insect midgut epithelial cells and their knockdown is lethal to insect larvae. To investigate the RNAi response mediated by multiple siRNA duplexes, partial length cDNA of Helicoverpa armigera coatomer beta and v-ATPase A was cloned and siRNA feeding-based RNAi was performed. Simultaneous ingestion of siRNAs specific to the H. armigera coatomer beta and v-ATPase A led to co-silencing of the target genes and reduction in larval survival rate and weight gain. These results suggest that silencing two genes by feeding of multiple siRNAs is a good RNAi strategy. (C) 2014 Elsevier Inc. All rights reserved.
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