您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Overexpression of tomato mitogen-activated protein kinase SlMPK3 in tobacco increases tolerance to low temperature stress

文献类型： 外文期刊

作者： Yu, Li 1 ; Yan, Jun 1 ; Yang, Yanjuan 2 ; Zhu, Weimin 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

2.Guangxi Univ, Coll Agr, Dept Hort Sci, Nanning 530004, Peoples R China

关键词： Tomato;MAPK;SlMPK3;Low temperature stress;Reactive oxygen species;Transgenic tobacco

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Mitogen-activated protein kinase (MAPK) cascades are important intracellular signaling events that mediate signal transduction associated with plant responses to environmental stresses. In this study, tomato plants were subjected to treatments of low (4 A degrees C) and high-temperature (42 A degrees C) and exogenous application of methyl jasmonate, salicylic acid and hydrogen peroxide. Results show that tomato leaves from treated plants contained an elevated level of the MAPK gene (SlMPK3). Transient expression assay of onion epidermal cells using pBI121-GFP constructs indicate that the SlMPK3 was predominantly localized in nuclei. In transgenic tobacco plants overexpressing SlMPK3, leaves contained less reactive oxygen species (ROS), more superoxide dismutase, peroxidase, catalase, ascorbate peroxidase activities, higher proline and soluble sugar contents, and an elevated transcript level of stress-responsive genes. Seeds derived from those SlMPK3 transgenic tobacco plants had a significant higher germination rate (P < 0.05) under chilling stress (12 A degrees C). The transgenic seedlings also developed larger root system under the same temperature treatment conditions. Taken together, these data demonstrate that SlMPK3 acts as a positive regulator in the response to low temperature stress by regulating ROS homeostasis through activation of cellular antioxidant systems and modulating the transcription of stress-associated genes.

  • 相关文献

[1]Enhanced Tolerance to Chilling Stress in Tomato by Overexpression of a Mitogen-Activated Protein Kinase, SlMPK7. Yu, Li,Yan, Jun,He, Lizhong,Zhu, Weimin,Yang, Yanjuan.

[2]Root cultures for elucidating tomato root reactions to NaCl stress. K. Kouki,A. Mougou,R. Paul. 2001

[3]Oral immunization of mice with plant-derived fimbrial adhesin FaeG induces systemic and mucosal K88ad enterotoxigenic Escherichia coli-specific immune responses. Liang, WQ,Huang, YH,Yang, XH,Zhou, Z,Pan, AH,Qian, BJ,Huang, C,Chen, JX,Zhang, DB. 2006

[4]Invasive ornamental plants: Problems, challenges, and molecular tools to neutralize their invasiveness. Li, Y,Cheng, ZM,Smith, WA,Ellis, DR,Chen, YQ,Zheng, XL,Pei, Y,Luo, KM,Zhao, DG,Yao, QH,Duan, H,Li, Q.

[5]Isolation and characterization of BnMKK1 responsive to multiple stresses and affecting plant architecture in tobacco. Yu, Shunwu,Chen, Chen,Li, Jiajia,Ye, Shuifeng,Liu, Guolan,Mei, Xiaohan,Luo, Lijun,Zhang, Lida,Tang, Kexuan.

[6]Overexpression of a bacterial mercury transporter MerT in Arabidopsis enhances mercury tolerance. Xu, Sheng,Sun, Bin,Wang, Rong,He, Jia,Xia, Bing,Wang, Ren,Xu, Sheng,Sun, Bin,Wang, Rong,He, Jia,Xia, Bing,Wang, Ren,Xue, Yong.

[7]Amelioration of salt-induced oxidative stress in eggplant by application of 24-epibrassinolide. Ding, H-D,Zhu, Z-W,Yang, S-J,Zha, D-S,Wu, X-X,Ding, H-D,Zhu, X-H,Wu, X-X.

[8]Stress responses to trichlorophenol in Arabidopsis and integrative analysis of alteration in transcriptional profiling from microarray. Li, Zhenjun,Zhu, Bo,Wang, Bo,Gao, Jianjie,Fu, Xiaoyan,Yao, Quanhong.

[9]Heat shock factors in tomatoes: genome-wide identification, phylogenetic analysis and expression profiling under development and heat stress. Yang, Xuedong,Zhu, Weimin,Zhang, Hui,Liu, Na,Tian, Shoubo. 2016

[10]Soil salinity increases the tolerance of excessive sulfur fumigation stress in tomato plants. Ding, Xiaotao,Ding, Xiaotao,Deng, Qi,Yu, Chih-Li,Hu, Dafeng, I,Zhang, Dong,Jiang, Yuping,Zhou, Suping.

[11]Different effects on ACC oxidase gene silencing triggered by RNA interference in transgenic tomato. Xiong, AS,Yao, QH,Peng, RH,Li, X,Han, PL,Fan, HQ. 2005

[12]Molecular characterization and infectivity of Papaya leaf curl China virus infecting tomato in China. Zhang, Hui,Ma, Xin-ying,Qian, Ya-juan,Zhou, Xue-ping,Zhang, Hui. 2010

[13]Genome-Wide Identification and Analysis of the MYB Transcription Factor Superfamily in Solanum lycopersicum. Li, Zhenjun,Peng, Rihe,Tian, Yongsheng,Han, Hongjuan,Xu, Jing,Yao, Quanhong.

[14]Infectivity of Tomato Leaf Curl Hainan Virus in Tomato and Tobacco in China. Zhang, Hui,Tian, Shoubo,Wu, Xuexia,Liu, Longzhou,Liu, Na,Zhu, Weimin. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台