Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction
文献类型: 外文期刊
作者: Zhang, Yan-Min 1 ; Zhang, Hong-Mei 1 ; Liu, Zi-Hui 1 ; Guo, Xiu-Lin 1 ; Li, Hui-Cong 1 ; Li, Guo-Liang 1 ; Jiang, Chun 1 ;
作者机构: 1.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Food & Oil Crops, Shijiazhuang 050035, Peoples R China
关键词: Soybean;Isoflavones;Inhibition;Transformation;Agrobacterium tumefaciens
期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )
ISSN:
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收录情况: SCI
摘要: Soybean is a difficult crop to manipulate through Agrobacterium tumefaciens-mediated genetic transformation. Plant-bacterium interaction plays an important role in the transformation process. Being rich with isoflavones, soybean may have an adverse effect on the A. tumefaciens-mediated genetic transformation. To investigate whether high content of endogenous isoflavones in soybean is a serious obstacle in achieving high efficient Agrobacterium-mediated transformation in soybean, a series of experiments on inhibition of isoflavone biosynthesis were conducted to improve upon soybean transformation efficiency. Results indicated that soybean isoflavones inhibited A. tumefaciens growth and respiration, the transformation efficiency [beta-glucuronidase (GUS) transient expression] was negatively correlated with the phenylalanine ammonia-lyase activity and isoflavones content. The biosynthesis of soybean isoflavones was partially inhibited by sonication treatment and applying antagonists in co-culture medium and thereby decreased the adverse effects of isoflavones on Agrobacterium infection. A discernible improvement in transformation efficiency was achieved when sonication at 40 kHz for 3 min was applied along with Agro-infection and the explants were cultured on co-culture medium containing 20 mu M alpha-aminooxyacetic acid (AOA), with the percentage of GUS transient expression as 41.4 %, being 3.6 times higher than that not sonicated and co-cultured on medium without AOA. Sonication was found not only to simply make micro-wounds for Agrobacterium to penetrate or releasing phenolic compounds for induced Agrobacterium vir gene expression; it disturbed the biosynthesis of isoflavones at the transcription level and decreased the adverse effects of isoflavones on soybean transformation, and thereby improving soybean transformation efficiency.
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