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A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector

文献类型： 外文期刊

作者： Li, Shuo 1 ; Wang, Xi 1 ; Xu, Jianxiang 2 ; Ji, Yinghua 1 ; Zhou, Yijun 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Tech Serv, Ctr Diag & Detect Plant Virus Dis, Nanjing 210014, Jiangsu, Peoples R China

2.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Peoples R China

关键词： Rice stripe virus;Rice black-streaked dwarf virus;Simultaneous detection via RT-PCR

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

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收录情况： SCI

摘要： Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Bacterial microbiota in small brown planthopper populations with different rice viruses. Li, Shuo,Zhou, Changwei,Chen, Guangyi,Zhou, Yijun. 2017

[2]A novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. Ren, Chunmei,Cheng, Zhaobang,Yang, Liu,Miao, Qian,Fan, Yongjian,Zhou, Yijun.

[3]Dual transcriptome analysis reveals insights into the response to Rice black-streaked dwarf virus in maize. Zhou, Yu,Duan, Canxing,Hao, Zhuanfang,Li, Mingshun,Yong, Hongjun,Zhang, Degui,Zhang, Shihuang,Weng, Jianfeng,Li, Xinhai,Zhou, Yu,Xu, Zhennan,Wang, Zhenhua,Chen, Yanping,Meng, Qingchang,Wu, Jirong.

[4]Whole-genome expression analysis of Rice black-streaked dwarf virus in different plant hosts and small brown planthopper. Xu, Qiufang,Ni, Haiping,Zhang, Jinfeng,Lan, Ying,Ren, Chunmei,Zhou, Yijun,Xu, Qiufang,Lan, Ying,Ren, Chunmei,Zhou, Yijun,Ni, Haiping.

[5]Development and use of three monoclonal antibodies for the detection of rice black-streaked dwarf virus in field plants and planthopper vectors. Wu, Jianxiang,Ni, Yuequn,Liu, Huan,Rao, Lixia,Zhou, Xueping,Zhou, Yijun. 2013

[6]RNA-dependent RNA polymerase 6 of rice (Oryza sativa) plays role in host defense against negative-strand RNA virus, Rice stripe virus. Jiang, Lin,Qian, Dan,Zheng, Hong,Meng, Lin-Yan,Wei, Chun-Hong,Li, Yi,Chen, Jie,Le, Wen-Jing,Zhou, Tong,Zhou, Yi-Jun.

[7]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[8]Analysis of rice stripe virus whole-gene expression in rice and in the small brown planthopper by real-time quantitative PCR. Li, S.,Li, X.,Sun, L.,Zhou, Y.,Li, S.,Sun, L.. 2012

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[10]Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy. Deng, Jinhua,Li, Shuo,Ji, Yinghua,Zhou, Yijun,Deng, Jinhua,Hong, Jian. 2013

[11]Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice stripe virus. Liu, Zhiyang,Li, Xuejuan,Sun, Feng,Zhou, Tong,Zhou, Yijun,Liu, Zhiyang,Li, Xuejuan,Sun, Feng,Zhou, Tong,Zhou, Yijun. 2017

[12]Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Xiong, Ruyi,Wu, Jianxiang,Zhou, Xueping,Zhou, Yijun. 2009

[13]Arabidopsis is Susceptible to Rice stripe virus Infections. Sun, Feng,Yuan, Xia,Zhou, Tong,Fan, Yongjian,Zhou, Yijun,Yuan, Xia. 2011

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