您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A gold nanoparticle based immunosorbent bio-barcode assay combined with real-time immuno-PCR for the detection of polychlorinated biphenyls

文献类型： 外文期刊

作者： Yang, Guangxin 1 ; Zhuang, Huisheng 1 ; Chen, Hanyu 1 ; Ping, Xianyin 1 ; Bu, Dan 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China

2.Chinese Acad Fishery Sci, Aquat Prod Qual Inspect & Test Ctr Shanghai, East China Sea Fisheries Res Inst,Minist Agr, Lab Qual & Safety Risk Assessment Aquat Prod Shan, Shanghai 200090, Peoples R China

关键词： Gold nanoparticle;PCBs;Real-time immuno-PCR;Signal DNA

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A gold nanoparticle based bio-barcode assay combined with real-time immuno-polymerase chain reaction (PCR) was developed for the determination of polychlorinated biphenyls (PCBs). Gold nanoparticles modified with thiol-capped DNA and antibody for affixing probes were used to replace antibody-DNA conjugates in immuno-PCR. The modified gold nanoparticles were fixed on the PCR tube wall through a competitive immune reaction. Signal-DNA could be released from the gold nanoparticle surface into the tubes and used as template DNA. Real-time PCR was subsequently performed to directly quantify DNA signal. A detection limit of 2.55 pg L-1 was obtained under optimized conditions. This method was successfully used to detect Aroclor 1248 in Hairtail samples collected from the East China Sea near Zhejiang Province. Recovery values ranged from 84% to 104%, and the coefficient of variation (CV) was acceptable. Results were validated by GC-ECD, indicating that this convenient and sensitive method has great potential for trace PCBs screening in real environmental samples. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]南海北部沿岸牡蛎体内PCBs的时空分布特征及评价. 王清云,甘居利,陈海刚,马胜伟,张喆,蔡文贵,贾晓平. 2013

[2]Polychlorinated biphenyls and organochlorine pesticides in surface sediments from the sand flats of Shuangtaizi Estuary, China: levels, distribution, and possible sources. Yuan, Xiutang,Yang, Xiaolong,Na, Guangshui,Zhang, Anguo,Liu, Guize,Wang, Lili,Zhang, Anguo,Mao, Yuze,Li, Xiaodong.

[3]Residual Distribution and Risk Assessment of Polychlorinated Biphenyls in Surface Sediments of the Pearl River Delta, South China. Lai, Zini,Li, Xiuli,Li, Haiyan,Zhao, Lina,Zeng, Yanyi,Wang, Chao,Gao, Yuan,Liu, Qianfu,Lai, Zini,Li, Xiuli,Li, Haiyan,Zhao, Lina,Zeng, Yanyi,Wang, Chao,Gao, Yuan,Liu, Qianfu,Li, Xiuli,Zhao, Lina.

[4]Bioaccessibility of polychlorinated biphenyls in workplace dust and its implication for risk assessment. Kang, Yuan,Li, Laisheng,Zhang, Qiuyun,Zeng, Lixuan,Luo, Jiwen,Kang, Yuan,Man, Yubon,Wong, Ming Hung,Kang, Yuan,Man, Yubon,Wong, Ming Hung,Yin, Yi.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台