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Conserved and novel heat stress-responsive microRNAs were identified by deep sequencing in Saccharina japonica (Laminariales, Phaeophyta)

文献类型: 外文期刊

作者: Liu, Fuli 1 ; Wang, Wenjun 1 ; Sun, Xiutao 1 ; Liang, Zhourui 1 ; Wang, Feijiu 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词: miRNA;q-PCR;RNA-Seq;sRNA

期刊名称:PLANT CELL AND ENVIRONMENT ( 影响因子:7.228; 五年影响因子:7.791 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: As a temperate-cold species, Saccharina japonica often suffers heat stress when it is transplanted to temperate and subtropical zones. Study the heat stress response and resistance mechanism of Saccharina is of great significance for understanding the acclimation to heat stress under domestication as well as for breeding new cultivars with heat stress resistance. In this study, we identified a set of heat stress-responsive miRNAs and analysed their regulation during the heat stress response. CO (control) and heat stress (HS) sRNA libraries were constructed and sequenced. Forty-nine known miRNAs and 75 novel miRNAs were identified, of which seven known and 25 novel miRNAs were expressed differentially under heat stress. Quantitative PCR of six selected miRNAs confirmed that these loci were responsive to heat stress. Thirty-nine and 712 genes were predicted to be targeted by the seven known miRNAs and 25 novel miRNAs, respectively. Gene function and pathway analyses showed that these genes probably play important roles in S.japonica heat stress tolerance. The miRNAs identified represent the first set of heat-responsive miRNAs identified from S.japonica, and their identification can help elucidate the heat stress response and resistance mechanisms in S.japonica.

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