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Extraction optimization and biological properties of a polysaccharide isolated from Gleoestereum incarnatum

文献类型: 外文期刊

作者: Zhang, Zuo-fa 1 ; Lv, Guo-ying 1 ; Jiang, Xue 2 ; Cheng, Jian-hui 1 ; Fan, Lei-fa 1 ;

作者机构: 1.Zhejiang Acad Agr Sci, Inst Hort, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China

关键词: Polysaccharide;Gleoestereum incarnatum;Antioxidant activity;Antitumor activity

期刊名称:CARBOHYDRATE POLYMERS ( 影响因子:9.381; 五年影响因子:8.678 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Extraction was optimized of polysaccharides from Gleoestereum incarnatum (GIP). The three parameters, extraction temperature, extraction time and the ratio of water to raw material, were optimized using the Box-Behnken design. As a result, the optimal extraction conditions were: extraction temperature 87.5 degrees C, extraction time 1 h and the ratio of water to raw material of 39.7 mL/g, where the highest yield of polysaccharide (13.18%) was obtained. GIP-II was the main fraction purified form GIP. GIP-II was composed of galactose, glucose, xylose, and mannose, with glucose was the predominant monosaccharide. GIP-II exhibited strong scavenging activities against DPPH and hydroxyl radials in vitro, as well as a strong inhibitory effect on the growth of HepG2 cells. The overall findings indicated that GIP-II is worthy of further exploration for its potential applications in antitumor drugs or health foods. (C) 2014 Elsevier Ltd. All rights reserved.

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