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Structural Characterization and Anticancer Activity of Cell-Bound Exopolysaccharide from Lactobacillus helveticus MB2-1

文献类型： 外文期刊

作者： Li, Wei 1 ; Xia, Xiudong 2 ; Tang, Weizhi 1 ; Ji, Juan 1 ; Rui, Xin 1 ; Chen, Xiaohong 1 ; Jiang, Mei 1 ; Zhou, Jianzhong 2 ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210095, Jiangsu, Peoples R China

关键词： Lactobacillus helveticus MB2-1;cell-bound exopolysaccharide (c-EPS);structural characterization;anticancer activity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： A novel cell-bound exopolysaccharide (c-EPS) was isolated from Lactobacillus helveticus MB2-1 by ultrasonic extraction, anion exchange, and gel filtration chromatography before being structurally characterized. The c-EPS is a heteropolysaccharide with an average molecular weight of 1.83 x 10(5) Da and is composed of glucose, mannose, galactose, rhamnose, and arabinose at a molar ratio of 3.12:1.01:1.00:0.18:0.16. Methylation analysis and nuclear magnetic resonance analysis revealed that the c-EPS is a linear glucomannogalactan containing repeating units of -> 6)-beta-d-Manp-(1 -> 3)-beta-d-Glcp-(1 -> 3)-beta-d-Glcp-(1 -> 3)-beta-d-Glcp-(1 -> 4)-alpha-d-Galp-(1 -> and trace amounts of Rhap-(1 -> and (1 -> 4)-Arap residues. Complex formation with Congo red demonstrated a triple-strand helical conformation for the c-EPS. Scanning electron microscopy of the c-EPS revealed many regular feather-like structural units. Topographical examination of c-EPS by atomic force microscopy revealed that the c-EPS formed rounded-to-spherical lumps with different sizes and chain formations. Furthermore, preliminary in vitro tests revealed that c-EPS significantly inhibited the proliferation of HepG-2, BGC-823, and especially HT-29 cancer cells.

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[1]Characterization of a novel polysaccharide with anti-colon cancer activity from Lactobacillus helveticus MB2-1. Li, Wei,Tang, Weizhi,Ji, Juan,Rui, Xin,Chen, Xiaohong,Jiang, Mei,Dong, Mingsheng,Xia, Xiudong,Zhou, Jianzhong.

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