您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An electrochemical deoxyribonucleic acid biosensor for rapid genotoxicity screening of chemicals

文献类型： 外文期刊

作者： Wu, Lidong 2 ; Lu, Xianbo 1 ; Wang, Xue 1 ; Song, Yi 2 ; Chen, Jiping 1 ;

作者机构： 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

2.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

期刊名称：ANALYTICAL METHODS （ 影响因子：2.896； 五年影响因子：2.716 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A sensitive electrochemical biosensor based on double-stranded deoxyribonucleic acid (DNA) has been proposed for rapid screening of chemicals genotoxicity potential. A DNA probe from the clone RP3-402G11 gene of the human DNA sequence and electroactive methylene blue (MB) have been used as a biorecognition element and signal amplification molecules respectively for evaluating the genotoxic potential of target analytes with high sensitivity. The biosensing mechanism of genotoxicity screening is based on the damage of targets for the DNA double helix, which results in the subsequent distinct change of the electrochemical signal. More than 10 kinds of genotoxic chemicals have been used as testing analytes including highly toxic dioxins (polychlorinated dibenzodioxins, polychlorinated dibenzofurans) and polychlorinated biphenyls (PCBs). Dioxins and dioxin-like chemicals have been identified as highly genotoxic chemicals by the proposed DNA biosensor, which is consistent with the conclusion from International Agency for Research on Cancer. The results obtained demonstrated that the signal response of the biosensor for dioxins and PCBs correlated well with their toxic equivalent factor (TEF) values and concentrations of tested targets. The biosensor proved to be a promising in vitro screening tool for rapid estimation of chemicals genotoxicity potential.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台