文献类型： 外文期刊

作者： Zou, Linyou ¹ ; Ross, Brian M. ² ; Hutchison, Leonard J. ³ ; Christopher, Lew P. ⁴ ; Dekker, Robert F. H. ⁴ ; Malek ¹ ;

作者机构： 1.Qinghai Acad Agr & Forestry Sci, Xining 810016, Qinghai, Peoples R China

2.Lakehead Univ, Northern Ontario Sch Med, Thunder Bay, ON P7B 5E1, Canada

3.Lakehead Univ, Fac Nat Resources Management, Thunder Bay, ON P7B 5E1, Canada

4.Lakehead Univ, Biorefining Res Inst, Thunder Bay, ON P7B 5E1, Canada

5.Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, Canada

关键词： Lignin demethylation;Fungal culture;Methanol mass spectrometry;Vicinal hydroxyl assay;HPLC

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.493； 五年影响因子：3.699 ）

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收录情况： SCI

摘要： Demethylation of industrial lignin has been for long coveted as a pathway to the production of an abundant natural substitute for fossil-oil derived phenol. In an attempt to possibly identify a novel Kraft lignin-demethylating enzyme, we surveyed a collection of fungi by using selected ion flow tube-mass spectrometry (SIFT-MS). This method readily identifies methanol resulting from lignin demethylation activity. Absidia cylindrospora, and unidentified Cylindrocladium sp. and Aspergillus sp. were shown to metabolize lignin via different pathways, based on the HPLC analysis of lignin fragments. Of these three, Cylindrocladium and Aspergillus were shown to retain most of the lignin intact after 3 weeks in culture, while removing about 40% of the available methoxy groups. Our results demonstrate that after optimization of culture and lignin recovery methods, biological modification of Kraft lignin may be a feasible pathway to obtaining demethylated lignin for further industrial use. Crown Copyright (c) 2015 Published by Elsevier Inc. All rights reserved.