您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cloning and characterization of calreticulin and its association with salinity stress in P-trituberculatus

文献类型： 外文期刊

作者： Lv, Jianjian 1 ; Wang, Yu 1 ; Zhang, Dening 1 ; Gao, Baoquan 1 ; Liu, Ping 1 ; Li, Jian 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Col

关键词： Portunus trituberculatus;Calreticulin;Salinity;Single nucleotide polymorphism

期刊名称：CELL STRESS & CHAPERONES （ 影响因子：3.667； 五年影响因子：3.717 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Calreticulin (CRT) is a highly conserved and multifunctional endoplasmic reticulum (ER) chaperone protein and plays important roles in salinity stress response. Portunus trituberculatus is a commercially important fishery species, and water salinity conditions influence its commercial farming significantly. In order to research the function of calreticulin under salinity stress, the full-length cDNA sequence of calreticulin from P. trituberculatus (PtCRT) was firstly cloned and characterized. The complete cDNA sequence of PtCRT is 1676 bp with 1218 bp open reading frame (ORF), encoding a polypeptide of 405 amino acids. Multiple sequence alignments showed that the deduced acid amino sequences of PtCRT shared the highest homology to CRT of Fenneropenaeus chinensis (89 %). Fluorescent quantitative real-time PCR analysis indicated that PtCRT was expressed in all detected tissues and showed the highest expression level in hepatopancreas. In addition, salinity challenge significantly influenced the expression level of PtCRT in gill. Six single nucleotide polymorphisms (SNPs) were detected in cDNA sequence of PtCRT, and one SNP was associated with the salt tolerant trait. All results indicated that PtCRT plays an important role in mediating the salinity adaption of P. trituberculatus.

  • 相关文献

[1]Na+/K+-ATPase alpha-subunit in swimming crab Portunus trituberculatus: molecular cloning, characterization, and expression under low salinity stress. Han, Xiaolin,Liu, Ping,Gao, Baoquan,Wang, Haofeng,Duan, Yafei,Xu, Wenfei,Chen, Ping,Han, Xiaolin. 2015

[2]The identification and characteristics of salinity-related microRNAs in gills of Portunus trituberculatus. Li, Jian.

[3]Identification and characterization of a novel calreticulin involved in the immune response of the Zhikong scallop, Chlamys farreri. Wang, Guanghua,Zhu, Dongfa,Wang, Guanghua,Yang, Ning,Zhang, Min,Jiang, Zengjie,Jiang, Zengjie.

[4]Current fishing practices may induce low risk of sperm limitation in female swimming crab Portunus trituberculatus in the East China Sea. Xuan, Fujun,Tang, Boping,Zhou, Chunlin,Guan, Weibing,Bao, Chengman,Tang, Fenghua.

[5]Effect of inbreeding on growth and genetic diversity of Portunus trituberculatus based on the full-sibling inbreeding families. Gao, Baoquan,Wang, Qingyin,Gao, Baoquan,Liu, Ping,Li, Jian,Wang, Qingyin,Han, Zhike.

[6]iTRAQ-based identification of differentially expressed proteins related to growth in the swimming crab, Portunus trituberculatus. Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Li, Jian,Liu, Ping,Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Li, Jian,Liu, Ping,Yu, Xuan.

[7]Effects of florfenicol on the antioxidant status, detoxification system and biomolecule damage in the swimming crab (Portunus trituberculatus). Ren, Xianyun,Wang, Zhuqing,Gao, Baoquan,Liu, Ping,Li, Jian,Ren, Xianyun,Wang, Zhuqing,Gao, Baoquan,Liu, Ping,Li, Jian,Wang, Zhuqing.

[8]Amino Acid, Fatty Acid, and Metal Compositions in Edible Parts of Three Cultured Economic Crabs: Scylla paramamosain, Portunus trituberculatus, and Eriocheir sinensis. Jiang, Ke-Ji,Zhang, Feng-Ying,Zhang, Dan,Sun, Man-Man,Gao, Lu-Jiao,Qiao, Zhen-Guo,Ma, Ling-Bo,Pi, Yan,Jiang, Li-Li,Yu, Zhong-Li,Zhang, Dan,Sun, Man-Man.

[9]Genetic diversity of different populations and improved growth in the F1 hybrids in the swimming crab (Portunus trituberculatus). Gao, B. Q.,Liu, P.,Li, J.,Wang, Q. Y.,Li, X. P.,Gao, B. Q.. 2014

[10]Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus. Wang, Qingyin. 2010

[11]cDNA cloning and characterization of peroxiredoxin gene from the swimming crab Portunus trituberculatus. Li, Jian. 2011

[12]White spot syndrome virus quantification in blue crab Portunus trituberculatus hatchery-produced larvae and wild populations by TaqMan real-time PCR, with an emphasis on the relationship between viral infection and crab health. Meng, Xian-Hong,Jang, In-Kwon,Seo, Hyung-Chul,Cho, Yeong-Roc,Meng, Xian-Hong.

[13]Molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab Portunus trituberculatus. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[14]Eighteen novel polymorphic microsatellites for the swimming crab (Portunus trituberculatus). Dong, Zhiguo,Sun, Xiaowen,Dong, Zhiguo,Dong, Zhiguo,Li, Xiaoying,Yan, Binlun.

[15]The immune responses and antioxidant status of Portunus trituberculatus individuals with different body weights. Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Li, Jian,Liu, Ping,Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Li, Jian,Liu, Ping,Yu, Xuan.

[16]Immune responses and expression of immune-related genes in swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N stress. Yue, Feng,Pan, Luqing,Xie, Peng,Zheng, Debin,Li, Jian.

[17]The cytosolic manganese superoxide dismutase cDNA in swimming crab Portunus trituberculatus: Molecular cloning, characterization and expression. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[18]Characterization of three caspases and their pathogen-induced expression pattern in Portunus trituberculatus. Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Liu, Ping,Li, Jian,Ren, Xianyun,Yu, Xuan,Gao, Baoquan,Liu, Ping,Li, Jian,Yu, Xuan.

[19]cDNA cloning, characterization and expression analysis of catalase in swimming crab Portunus trituberculatus. Chen, Ping,Li, Jitao,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian,Chen, Ping,Li, Jitao,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[20]Variation of antioxidant/detoxification enzyme activities in response to benzo[a]pyrene in the gazami crab Portunus trituberculatus. Li, Lei,Jiang, Mei,Shen, Xinqiang.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台