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O-2 '-Hydroxypropyltrimethyl ammonium chloride chitosan nanoparticles for the delivery of live Newcastle disease vaccine

文献类型： 外文期刊

作者： Dai, Chunxiao 1 ; Kang, Hong 1 ; Yang, Wanqiu 3 ; Sun, Jinyan 4 ; Liu, Chunlong 5 ; Cheng, Guogang 2 ; Rong, Guangyu 1 ;

作者机构： 1.Heilongjiang Univ, Sch Life Sci, Key Lab Microbiol, Harbin 150080, Peoples R China

2.Heilongjiang Univ, Key Lab Chem Engn Proc & Technol High Efficiency, Harbin 150080, Peoples R China

3.Harbin Pharmaceut Grp Biovaccine Co Ltd, Harbin 150069, Peoples R China

4.Heilongjiang Acad Agr Sci, Anim Husb Res Inst, Harbin 150086, Peoples R China

5.Chinese Acad Sci, Northeast Inst Geog & Agr Ecol, Harbin 150081,

关键词： Nanoparticles;Chitosan derivatives;Newcastle disease;Mucosal immune delivery system;Sustained release

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：9.381； 五年影响因子：8.678 ）

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收录情况： SCI

摘要： A novel complex chitosan derivative, O-2'-hydroxypropyltrimethyl ammonium chloride chitosan (O-2'-HACC), was synthesized and used to make nanoparticles as a delivery vehicle for live attenuated Newcastle disease vaccine. We found that O-2'-HACC had high antimicrobial activity, low toxicity, and a high safety level. Newcastle disease virus (NDV) was then encapsulated in the O-2'-HACC nanoparticles (NDV/La Sota-O-2'-HACC-NPs) by the ionic crosslinking method, and the properties of the resulting nanoparticles were determined by transmission electron microscopy, Zeta potential analysis, Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, and X-ray diffraction. NDV/La Sota-O-2'-HACC-NPs had regular spherical morphologies and high stability, with an encapsulation efficiency of 95.68 +/- 2.2% and a loading capacity of 58.75 +/- 4.03%. An in vitro release assay indicated that release of NDV from NDV/La Sota-O-2'-HACC-NPs occurred slowly. Specific pathogen-free chickens immunized with NDV/La Sota-O-2'-HACC-NPs intranasally had much stronger cellular, humoral and mucosal immune responses than did those immunized intramuscularly or with live attenuated Newcastle disease vaccine. NDV/La Sota-O-2'-HACC-NPs are a novel drug delivery carrier with immense potential in medical applications. (C) 2015 Elsevier Ltd. All rights reserved.

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