Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa
文献类型： 外文期刊
作者： Zhang, Jiewei 1 ; Zhang, Zhongbao 1 ; Zhu, Dan 1 ; Guan, Yang 1 ; Shi, Dianyi 2 ; Chen, Yajuan 1 ; Li, Ruifen 1 ; Wang, H 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China
关键词： PI-PLC;Pto PLC1;Populus tomentosa;qReal-time PCR;Salt stress
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.175； 五年影响因子：1.238 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Phosphoinositide-specific phospholipase C (PI-PLC) is a critical enzyme family, participating in various signal-transduction pathways in eukaryotic cells. However, little information on PI-PLC in plants is available. In this study, a PI-PLC gene, PtoPLC1 was obtained from Populus tomentosa and initial characterized. The recombinant PtoPLC1 protein expressed in vitro was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The catalysis of PIP2 by PtoPLC1 is Ca2+ dependent and the optimum concentration of Ca2+ is 1 mu M. These results suggested that PtoPLC1 gene encodes a functional PI-PLC. The qReal-time PCR analysis revealed strong expression of PtoPLC1 in roots, but weak expression in stems and leaves. The gene is strongly induced under high salt, dehydration and ABA. These results suggest that PtoPLC1 might be involved in the signal-transduction pathways of high salt, dehydration and ABA responses in P. tomentosa.
- 相关文献
作者其他论文 更多>>
-
Insights into Molecular Mechanism of Secondary Xylem Rapid Growth in Salix psammophila
作者：Qiao, Hongxia;Wei, Dongshan;Li, Yingjie;Chao, Kairui;Yang, Haifeng;Qiao, Hongxia;Wang, Yunhan;Liu, Guiming;Zhang, Jiewei;Qiao, Hongxia;Wang, Yunhan;Liu, Guiming;Zhang, Jiewei;Wang, Yunhan;Shi, Lin;Wang, Ruiping;Yang, Yeru;Jia, Li;Yu, Fengqiang
关键词：
Salix psammophila ; secondary growth; secondary xylem; lignin; caffeoyl shikimate esterase -
The TIFY transcription factor ZmJAZ13 enhances plant tolerance to drought and salt stress by interacting with ZmbHLH161 and ZmA0A1D6GLB9
作者：Zhang, Shipeng;Lu, Yuncai;Zhang, Shipeng;Zheng, Dengyu;Gao, Yuqi;She, Meng;Wu, Zhongyi;Zhang, Zhongbao;Gao, Yuqi
关键词：Maize; ZmJAZ13; Drought stress; Salt stress; Response to adversity
-
Integrative Physiological and Transcriptomic Analysis Reveals Metabolic Adaptation and Cold-Tolerance Marker Development in Winter Rye Under Low-Temperature Stress
作者：Li, Haonan;Zhao, Jiahuan;He, Chenguang;Guan, Huimin;He, Ting;Meng, Dexu;Wang, Xiaoping;Guan, Yang;Tang, Yimiao
关键词：rye (
Secale cereale ); low-temperature stress; metabolism; molecular markers -
Quantitative proteomic and phosphoproteomic analyses reveal salt tolerance mechanisms of the halophyte Hordeum brevisubulatum
作者：Feng, Hao;Xiao, Fang;Jiang, Ying;Li, Ruifen;Zhao, Jielong;Lu, Yuncai;Xiao, Fang;Wang, Shuyue
关键词：Salt stress; Protein abundance; Protein phosphorylation; Dehydrin; Crop wild relative
-
The PeachSNP170K array facilitates insights into a large-scale population relatedness and genetic impacts on citrate content and flowering time
作者：Xu, Yaoguang;Yu, Yang;Qi, Xinpeng;Zhang, Qi;Guan, Jiantao;Zhang, Zhengquan;Wei, Jianhua;Xie, Hua
关键词：
-
Insight into the Functional Role of SiMPK6 in Stress Response and Photosynthetic Efficiency in Setaria italica
作者：Zhu, Dan;Hu, Xiaobing;Feng, Feng;Chai, Ran;Wang, Hailong;Li, Xianglong;Song, Wenqing;Wei, Jianhua;Zhang, Jiewei;Zhang, Yonghu;Wen, Rui
关键词：
Setaria italica ; MAPK protein; SiMPK6; foxtail millet; abiotic stresses; photosynthetic efficiency -
Exploring Transcriptional Regulatory Network During Direct De Novo Shoot Regeneration in Poplar
作者：Ding, Liping;Yang, Zitong;Zheng, Lin;Chen, Yajuan;Wei, Jianhua;Wang, Hongzhi
关键词：regeneration; hormone;
populus ; transcriptome
