Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa
文献类型： 外文期刊
作者： Zhang, Jiewei 1 ; Zhang, Zhongbao 1 ; Zhu, Dan 1 ; Guan, Yang 1 ; Shi, Dianyi 2 ; Chen, Yajuan 1 ; Li, Ruifen 1 ; Wang, H 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China
关键词： PI-PLC;Pto PLC1;Populus tomentosa;qReal-time PCR;Salt stress
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.175； 五年影响因子：1.238 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： Phosphoinositide-specific phospholipase C (PI-PLC) is a critical enzyme family, participating in various signal-transduction pathways in eukaryotic cells. However, little information on PI-PLC in plants is available. In this study, a PI-PLC gene, PtoPLC1 was obtained from Populus tomentosa and initial characterized. The recombinant PtoPLC1 protein expressed in vitro was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The catalysis of PIP2 by PtoPLC1 is Ca2+ dependent and the optimum concentration of Ca2+ is 1 mu M. These results suggested that PtoPLC1 gene encodes a functional PI-PLC. The qReal-time PCR analysis revealed strong expression of PtoPLC1 in roots, but weak expression in stems and leaves. The gene is strongly induced under high salt, dehydration and ABA. These results suggest that PtoPLC1 might be involved in the signal-transduction pathways of high salt, dehydration and ABA responses in P. tomentosa.
- 相关文献
作者其他论文 更多>>
-
Genome-wide identification and characterization of FAD family genes in barley
作者：Cao, Tingting;Ge, Rongchao;Du, Qingwei;Li, Ruifen
关键词：FAD; Barley; Gene family; Gene expression; Subcellular localization
-
Assembly and comparative analysis of the first complete mitochondrial genome of Setaria italica
作者：Zhang, Jiewei;Liu, Guiming;Wei, Jianhua
关键词：Comparative genomics; Foxtail millet; Mitochondrial genome; Phylogeny; Setaria italica
-
Functional identification and characterization of two flavonoid glycosyltransferases ZmUGT84A3 and ZmUGT84A4 from maize
作者：Ke, Zhao;Lu, Min;Ke, Zhao;Zheng, Dengyu;She, Meng;Zhang, Shipeng;Wu, Zhongyi;Zhang, Zhongbao;She, Meng
关键词：Uridine diphosphate glycosyltransferases; Maize; Flavonoid; Luteolin; In vitro experiments
-
Cloning, Expression, and Functional Characterization of Two Highly Efficient Flavonoid-di-O-glycosyltransferases ZmUGT84A1 and ZmUGT84A2 from Maize (Zea mays L.)
作者：Sun, Xiaorong;Ke, Zhao;Zheng, Dengyu;She, Meng;Wu, Zhongyi;Zhang, Zhongbao;Ke, Zhao;She, Meng;Li, Qing X.
关键词：flavonoid-di-O-glycosyltransferase; ZmUGT84A1; ZmUGT84A2; glycoxidation; glycoside; maize
-
The transcription factor MYB156 controls the polar stiffening of guard cell walls in poplar
作者：Zheng, Lin;Chen, Yajuan;Ding, Liping;Zhou, Ying;Xue, Shanshan;Li, Biying;Wei, Jianhua;Wang, Hongzhi
关键词：
-
Comparative genomics reveals a unique nitrogen-carbon balance system in Asteraceae
作者：Shen, Fei;Qin, Yajuan;Huang, Xin;Wang, Ying;Wei, Jianhua;Yang, Xiaozeng;Wang, Rui;He, Junna;Wang, Ying;Zhou, Yue;Li, Lei;Gao, Tiangang;Jiao, Yuannian
关键词：
-
LettuceGDB: The community database for lettuce genetics and omics
作者：Guo, Zhonglong;Li, Bo;Du, Jianjun;Shen, Fei;Zhao, Yongxin;Deng, Yang;Kuang, Zheng;Lu, Xianju;Wang, Ying;Wei, Jianhua;Guo, Xinyu;Zhao, Chunjiang;Yang, Xiaozeng;Guo, Zhonglong;Tao, Yihan;Wan, Miaomiao;Wang, Ying;Li, Lei;Guo, Zhonglong;Tao, Yihan;Wan, Miaomiao;Wang, Ying;Li, Lei;Guo, Zhonglong;Li, Bo;Shen, Fei;Zhao, Yongxin;Deng, Yang;Kuang, Zheng;Wang, Ying;Yang, Xiaozeng;Guo, Zhonglong;Du, Jianjun;Lu, Xianju;Guo, Xinyu;Zhao, Chunjiang;Wang, Dong;Han, Yingyan
关键词：lettuce; genome; multi-omics; germplasms; breeding; community
