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Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

文献类型： 外文期刊

作者： Yang, Xing-dong 1 ; Wang, Fang-yu 1 ; Song, Chun-mei 3 ; Wu, Shu-yan 3 ; Zhang, Gai-ping 1 ; Zeng, Xian-yin 2 ;

作者机构： 1.Henan Acad Agr Sci, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Peoples R China

2.Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China

3.Univ Shanghai Sci & Technol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450002, Peoples R China

关键词： Estradiol;Colloidal gold;Rapid detection

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：4.952； 五年影响因子：5.383 ）

ISSN：

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收录情况： SCI

摘要： It is a fact that the level of estradiol (E2) in powdered milk has been increased manyfold with growing concerns on dairy safety. Our study produced a panel of monoclonal antibodies (mAb) specific for E2 screening and established a lateral flow colloidal gold immunoassay strip for determining E2 in milk samples with the E2 mAb. The sensitivity of the test strip was found to be 180.42 +/- 3.17 pg/mL and the lower detection limit (LDL) value was 37.14 pg/mL. For negative milk samples spiked with 400, 800, and 1200 pg/mL, the recovery range was 86.7-93.5% and the coefficient of variation scope [CV (%)] was 4.8 -6.2%. The results of comparative studies between test strips and HPLC were relatively identical while the strip test only required less than 8 min. In the study, the test strip was a practical screening technology for quantitative, semi-quantitative, or qualitative detection of E2 residues. (C) 2015 Published by Elsevier Ltd.

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