MoS2 nanosheet-based fluorescent biosensor for protein detection via terminal protection of small-molecule-linked DNA and exonuclease III-aided DNA recycling amplification
文献类型： 外文期刊
作者： Xiang, Xia 1 ; Shi, Jianbin 2 ; Huang, Fenghong 1 ; Zheng, Mingming 1 ; Deng, Qianchun 1 ; Xu, Jiqu 1 ;
作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Dept Prod Proc & Nutriol,Key Lab Oil Crops Biol, Hubei Key Lab Lipid Chem & Nutr,Minist Agr, Wuhan 430062, Peoples R China
2.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan 430064, Hubei Province, Peoples R China
关键词： MoS2 nanosheet;Fluorescent biosensor;Protein detection;Terminal protection of small-molecule-linked DNA;Exonuclease III
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：10.618； 五年影响因子：9.323 ）
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收录情况： SCI
摘要： A new MOS2 nanosheet-based fluorescent biosensor for protein detection is developed. This method combines the terminal protection of small-molecule-linked DNA (TPSMLD) and exonuclease III (Exo III)aided DNA recycling amplification to convert protein assay into the highly sensitive detection of DNA. Taking the streptavidin (SA)-biotin system as a model, a detection limit of 0.67 ng mL(-1) SA is obtained with a good selectivity. The study demonstrated here not only offers simple, sensitive and selective detection method for protein assay, but also will expand the application of the emerging 2D nanomaterials into biological assay. (C) 2015 Elsevier B.V. All rights reserved.
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