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A sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Psathyrostachys huashanica confers abiotic stress tolerance in tobacco

文献类型： 外文期刊

作者： He, Xiaolan 1 ; Chen, Zhenzhen 1 ; Wang, Jianwei 2 ; Li, Wenxu 3 ; Zhao, Jixin 1 ; Wu, Jun 1 ; Wang, Zhonghua 1 ; Chen, X 1 ;

作者机构： 1.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China

2.Kaili Univ, Coll Environm & Life Sci, Kaili 556011, Guizhou, Peoples R China

3.Henan Acad Agr Sci, Inst Wheat Res, Zhengzhou 450002, Henan, Peoples R China

关键词： 6-SFT gene;Drought;Freezing;Psathyrostachys huashanica;Salinity;Transgenic tobacco

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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收录情况： SCI

摘要： Fructans are accessible carbohydrate reserves in various plant species, which possess many physiological functions including anti-oxidation, stabilizing subcellular structures, and osmotic adjustment. In addition, fructans may play important roles in stress tolerance in plant species. In this study, we isolated a Psathyrostachys huashanica (2n = 2x = 14, NsNs) sucrose:fructan-6-fructosyltransferase (Ph-6-SFT) using homologous cloning and genomic walking. Sequencing and gene structure analysis showed that Ph-6-SFT contains four exons and three introns, with a transcript of 2207 bp. Sequence analysis indicated that the coding sequence of Ph-6-SFT is 1851 bp long and it encodes 616 amino acids, where the structure shares high similarity with 6-SFTs from other plants. Furthermore, Ph-6-SFT was transferred into tobacco (Nicotiana tabacum L.) cv. W38 via Agrobacterium-mediated transformation. Compared with the wild-type plants, the transgenic tobacco plants exhibited a much higher tolerance of drought, cold, and high salinity. In all conditions, physiological studies showed that the tolerance of transgenic plants was associated with the accumulation of carbohydrate and proline, but reductions in malondialdehyde. Our results suggest that the 6-SFT gene from P. huashanica enhanced stress tolerance in tobacco plants and it may be applied as a genetic tool for improving stress tolerance in other crops. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]An intronless sucrose:fructan-6-fructosyltransferase (6-SFT) gene from Dasypyrum villosum enhances abiotic tolerance in tobacco. He, X. L.,Chen, Z. Z.,Wu, J.,Zhao, J. X.,Su, J. N.,Wang, Z. H.,Chen, X. H.,He, X. L.,Chen, X. H.,He, X. L.,Wang, J. W.,Li, W. X.. 2017

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