文献类型： 外文期刊

作者： Babu, Binoy ¹ ; Kefialew, Yonas W. ² ; Li, Ping-Fang ⁴ ; Yang, Xing-Ping ⁴ ; George, Sheeja ² ; Newberry, Eric ² ; Duf ¹ ;

作者机构： 1.Univ Florida, NFREC, Inst Food & Agr Sci, Quincy, FL 32351 USA

2.Univ Florida, NFREC, Gainesville, FL 32611 USA

3.Ethiopian Inst Agr Res, Gambella Agr Res Inst, Gambella, Ethiopia

4.Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing, Jiangsu, Peoples R China

5.Univ Florida, Inst Food & Agr Sci, Dept Plant Pathol, Gainesville, FL 32611 USA

6.Univ Addis Ababa, Dept & Microbial Cellular & Mol Biol, Addis Ababa, Ethiopia

7.Haramaya Univ, Sch Plant Sci, Dire Dawa, Ethiopia

期刊名称：PLANT DISEASE （ 影响因子：4.438； 五年影响因子：4.7 ）

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收录情况： SCI

摘要： Gummy stem blight caused by Didymella bryoniae (anamorph Phoma cucurbitacearum) is a major fungal disease of watermelon (Citrullus lanatus) and other cucurbits. Thirty-five isolates of Didymella and Phoma spp. associated with symptoms of gummy stem blight on watermelon, Canary melon {Cucumis melo), muskmelon (C. melo), and winter squash (Cucurbita maxima) from Florida and Georgia were characterized based on morphology on agar media, pathogenicity on 'Melody' watermelon, the internal transcribed spacer (ITS) sequence of ribosomal DNA (rDNA), random amplified polymorphic DNA (RAPD) analysis, and polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis. All of the isolateswere pathogenic on watermelon but differed in virulence. RAPD and ITS sequence analysis indicated genetic variability among the isolates but PCR-RFLP analysis did not show any variability. ITS sequence phy-logenetic analysis identified two isolates, DB-05 and DB-33, which had a greater identity to that of D. bryoniae isolates from China (98 to 100% sequence homology) than other isolates from Florida and Georgia (95 to 98%). These two isolates possessed a single nucleotide substitution of A to G at position 131 of the ITS 1 region. The study characterized the genetic profile of a collection of D. bryoniae isolates from Florida and Georgia in relation to isolates from other U.S. states and countries.