您好,欢迎访问江苏省农业科学院 机构知识库!

Molecular and functional characterization of the ecdysone receptor isoform-A from the cotton mirid bug, Apolygus lucorum (Meyer-Dur)

文献类型: 外文期刊

作者: Tan, Yong-An 1 ; Xiao, Liu-Bin 1 ; Zhao, Jing 1 ; Sun, Yang 1 ; Bai, Li-Xin 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

关键词: Apolygus lucorum;Ecdysone receptor;Expression profile;RNA interference

期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: We cloned the cDNA of the ecdysone receptor (EcR) isoform-A from the mind bug, Apolygus lucorum (AlEcR-A). The AlEcR-A cDNA has an open reading frame of 1410 bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its A/B-specific domain. Phylogenetic analysis showed that AlEcR-A is very similar to the EcR-A genes of other Hemiptera species. AlEcR-A mRNA was detected at all developmental stages of A. lucorum with peaks correlating to ecdysteroid pulses. AlEcR-A was also expressed in all analyzed tissues with maximum expression in the epidermis and fat body. An AlEcR-A mRNA of size 1.8 kb was detected in all tissues by northern blot analysis. We investigated the functions of AlEcR-A in A. lucorum growth and development using RNAi in vivo. Weights of fifth instar nymphs were significantly decreased in insects treated with AlEcR-A specific anti-sense RNA. Mortality from third instar nymphs to adults increased significantly along with a significant increase in instar duration. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Ecdysone receptor isoform-B mediates soluble trehalase expression to regulate growth and development in the mirid bug, Apolygus lucorum (Meyer-Dur). Tan, Y. -A.,Xiao, L. -B.,Zhao, J.,Sun, Y.,Bai, L. -X.,Xiao, Y. -F..

[2]MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES IN THE COTTON MIRID BUG, Apolygus lucorum. Tan, Yongan,Xiao, Liubin,Sun, Yang,Zhao, Jing,Bai, Lixin,Xiao, Yingfang.

[3]The nuclear hormone receptor E75A regulates vitellogenin gene (Al-Vg) expression in the mirid bug Apolygus lucorum. Tan, Y. -A.,Zhao, X. -D.,Hao, D. -J.,Sun, Y.,Zhao, J.,Jiang, Y. -P.,Bai, L. -X.,Xiao, L. -B.. 2018

[4]Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses. Zhao, Jing,Sheng, Yang,Bai, Li-Xin,Tan, Yongan,Xiao, Liu-Bin,Xu, Guang-Chun,Sun, Yang,Zhang, Yong-Jun,Xiao, Ying-Fang. 2016

[5]The AMT1 family genes from Malus robusta display differential transcription features and ammonium transport abilities. Li, Hui,Yang, Qing-song,Liu, Wei,Lin, Jing,Chang, You-hong.

[6]Characterization and expression profile of CaNAC2 pepper gene. Guo, Wei-Li,Chen, Ru-Gang,Yin, Yan-Xu,Gong, Zhen-Hui,Guo, Wei-Li,Chen, Bi-Hua,Du, Xiao-Hua,Zhang, Yu-Yuan,Wang, Shu-Bin. 2015

[7]RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens. Xu, Lu,Xu, De-Jin,Xu, Guang-Chun,Xu, Xiao-Long,Gu, Zhong-Yan,Xu, Lu,Zhao, Chun-Qing,Han, Zhao-Jun,Zhang, Ya-Nan. 2017

[8]Molecular evolution, characterization, and expression analysis of SnRK2 gene family in Pak-choi (Brassica rapa ssp chinensis). Huang, Zhinan,Tang, Jun,Duan, Weike,Wang, Zhen,Song, Xiaoming,Hou, Xilin,Tang, Jun. 2015

[9]Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress. Guo, Meng,Liu, Jin-Hong,Lu, Jin-Ping,Zhai, Yu-Fei,Wang, Hu,Gong, Zhen-Hui,Lu, Ming-Hui,Wang, Shu-Bin. 2015

[10]Genome-wide identification and characterization of GRAS transcription factors in sacred lotus (Nelumbo nucifera). Wang, Yu,Shi, Shenglu,Zhou, Ying,Zhou, Yu,Tang, Xiaoqing,Yang, Jie. 2016

[11]Validating the importance of two acetylcholinesterases in insecticide sensitivities by RNAi in Pardosa pseudoannulata, an important predatory enemy against several insect pests. Meng, Xiangkun,Li, Chunrui,Bao, Haibo,Liu, Zewen,Zhang, Yixi,Bao, Haibo,Fang, Jichao.

[12]Knockdown of NADPH-cytochrome P450 reductase results in reduced resistance to buprofezin in the small brown planthopper, Laodelphax striatellus (fallen). Zhang, Yueliang,Wang, Yaming,Wang, Lihua,Yao, Jing,Guo, Huifang,Fang, Jichao,Wang, Yaming.

[13]RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus. Fang, Ying,Lee, Seok Hee,Kim, Jong Hoon,Park, Dong Hwan,Park, Min Gu,Woo, Ra Mi,Lee, Bo Ram,Kim, Woo Jin,Je, Yeon Ho,Choi, Jae Young,Je, Yeon Ho,Li, Shuo. 2017

[14]Reduction of infectious bursal disease virus replication by shRNAs targeting the VP1 and VP2 genes driven by chicken U6 promoter. Wei Ouyang,Ma, Jin-rong,Jiang, Jie-yuan,Wang, Yong-shan,Wang, Yong-qiang,Qin, Li-ting,Wang, Xiao-mei,Fan, Hong-jie. 2013

[15]Resistance to cycloxaprid in Laodelphax striatellus is associated with altered expression of nicotinic acetylcholine receptor subunits. Zhang, Yueliang,Han, Yangchun,Yang, Qiong,Wang, Lihua,Guo, Huifang,Fang, Jichao,He, Peng,Liu, Zewen,Li, Zhong. 2018

作者其他论文 更多>>