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Development and characterization of microsatellite markers for the fruit fly, Bactrocera tau (Diptera: Tephritidae)

文献类型: 外文期刊

作者: Yan, Wei 1 ; Liu, Li 1 ; Huang, Shanchun 1 ; Li, Chaoxu 1 ; Ma, Zilong 1 ; Qin, Weiquan 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China

关键词: Bactrocera tau;Microsatellite;Polymorphism;Population genetics

期刊名称:APPLIED ENTOMOLOGY AND ZOOLOGY ( 影响因子:1.403; 五年影响因子:1.381 )

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收录情况: SCI

摘要: The fruit fly, Bactrocera tau (Walker), is one of the most important invasive worldwide pests on fruits and vegetables. In order to facilitate species identification and population genetic analysis, we isolated 22 microsatellite loci through an enriched genomic library protocol. Polymorphisms were evaluated in 30 individuals. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosities ranged from 0.048 to 0.862 and 0.136 to 0.852, respectively. The polymorphic information content values were from 0.124 to 0.818. Eight loci had significant deviations from the Hardy-Weinberg equilibrium. These SSR markers can be used to understand the population genetics and evolutionary patterns of the B. tau complex.

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