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Prophenoloxidase Response to White Spot Syndrome Virus Infection in the Red Swamp Crayfish (Procambarus clarkii)

文献类型： 外文期刊

作者： Li, Yanhe 1 ; She, Lei 1 ; Wu, Minglin 1 ; Wang, Yongjie 1 ; Ji, Suofei 1 ; Yang, Keli 3 ; Wang, Weimin 2 ;

作者机构： 1.Anhui Acad Agr Sci, Inst Fisheries, Hefei 230031, Anhui, Peoples R China

2.Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430064, Hubei, Peoples R China

期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：1.318； 五年影响因子：0.954 ）

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收录情况： SCI

摘要： Invertebrates rely on innate immunity to respond to the entry of foreign microorganisms. The prophenoloxidase (proPO) system plays crucial roles in crustacean innate immunity. White spot syndrome virus (WSSV) infection has recently been responsible for significant economic losses in many crayfish-producing farms in China. We therefore aimed to examine the response of the proPO system to WSSV infection. The virulence of the virus, expression of the proPO gene, and phenoloxidase (PO) activity in six tissues from the red swamp crayfish Procambarus clarkii were investigated using LD50 tests, real-time polymerase chain reaction and spectrophotometry, after infection with different numbers of WSSV virions. The LD50 of the WSSV strain was 2.08 x10(7) virions/crayfish. proPO mRNA expression was increased in all studied tissues after infection with WSSV, except in the cuticle epidermis after infection with 2 x 10(7) virions. proPO mRNA transcription was significantly increased in hemocytes and hepatopancreas, suggesting that P. clarkii might depend mainly on innate immunity for defense against viral pathogens. PO activity differed among different tissues during WSSV infection, suggesting that the proPO system might be activated by different mechanisms in different tissues in response to different viral stresses. (C) 2015 PVJ. All rights reserved

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