您好,欢迎访问中国水产科学研究院 机构知识库!

Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus)

文献类型: 外文期刊

作者: Jia, Rui 1 ; Han, Cen 3 ; Lei, Ji-Lin 2 ; Liu, Bao-Liang 2 ; Huang, Bin 2 ; Huo, Huan-Huan 1 ; Yin, Shu-Ting 2 ;

作者机构: 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China

3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

关键词: Nitrite;Oxidative stress;Apoptosis;Gills;Scophthalmus maximus

期刊名称:AQUATIC TOXICOLOGY ( 影响因子:4.964; 五年影响因子:5.071 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Nitrite (NO2-) is commonly present as contaminant in aquatic environment and toxic to aquatic organisms. In the present study, we investigated the effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Fish were exposed to various concentrations of nitrite (0, 0.02, 0.08, 0.4 and 0.8 mM) for 96 h. Fish blood and gills were collected to assay haematological parameters, oxidative stress and expression of genes after 0, 24, 48 and 96 h of exposure. In blood, the data showed that the levels of methemoglobin (MetHb), triglyceride (TG), potassium (K+), cortisol, heat shock protein 70 (HSP70) and glucose significantly increased in treatments with higher concentrations of nitrite (0.4 and/or 0.8 mM) after 48 and 96 h, while the levels of haemoglobin (Hb) and sodium (Na+) significantly decreased in these treatments. In gills, nitrite (0.4 and/or 0.8 mM) apparently reduced the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH), increased the formation of malondialdehyde (MDA), up-regulated the mRNA levels of c-jun amino-terminal kinase (JUK1), p53, caspase-3, caspase-7 and caspase-9 after 48 and 96 h of exposure. The results suggested caspase-dependent and JUK signaling pathways played important roles in nitrite-induced apoptosis in fish. Further, this study provides new insights into how nitrite affects the physiological responses and apoptosis in a marine fish. (C) 2015 Elsevier B.V. All rights reserved.

  • 相关文献

[1]The physiological performance and immune response of juvenile turbot (Scophthalmus maximus) to nitrite exposure. Jia, Rui,Lei, Ji-Lin,Han, Cen.

[2]Identification and mRNA expression of antioxidant enzyme genes associated with the oxidative stress response in the Wuchang bream (Megalobrama amblycephala Yih) in response to acute nitrite exposure. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Jiang, Xiaojun. 2014

[3]Effects of ammonia exposure on stress and immune response in juvenile turbot (Scophthalmus maximus). Jia, Rui,Lei, Ji-Lin,Liu, Bao-Liang,Huang, Bin,Han, Cen.

[4]Molecular cloning, immunohistochemical localization, characterization and expression analysis of caspase-8 from the blunt snout bream (Megalobrama amblycephala) exposed to ammonia. Sun, Shengming,Ge, Xianping,Zhu, Jian,Zhang, Wuxiao,Zhang, Qiong.

[5]Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Cheng, Chang-Hong,Guo, Zhi-Xun,Ye, Chao-Xia,Wang, An-Li,Guo, Zhi-Xun.

[6]Toxic dinoflagellate Alexandrium tamarense induces oxidative stress and apoptosis in hepatopancreas of shrimp (Fenneropenaeus chinensis). Liang Zhongxiu,Ren Hai,Liang Zhongxiu,Li Jian,Li Jitao,Tan Zhijun,Zhao Fazhen. 2014

[7]Effects of water-borne copper on the gills and hepatopancreas of Macrobrachium rosenbergii. Li, Na,Ma, Qiang,Zhao, Yunlong,Yang, Jian,Li, Na,Brochon, Jean-Claude. 2008

[8]Molecular cloning, characterization and expression analysis of gonadal P450 aromatase in the half-smooth tongue-sole, Cynoglossus semilaevis. Deng, Si-Ping,Chen, Song-Lin,Xu, Jian-Yong,Liu, Ben-Wei,Deng, Si-Ping.

[9]Molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster Pinctada fucata. Xu, Xinping,Xu, Xinping,Zhang, Dianchang,Jiang, Shigui,Ma, Jianjun,Jiang, Jingjing,Pan, Dequan,Zhang, Dianchang,Jiang, Jingjing.

[10]Optimization of nitrite reductase production conditions in Lactobacillus plantarum from salted fish. Wang, Yanan,Wu, Yanyan,Yang, Xianqing,Li, Laihao,Qi, Bo. 2013

[11]Molecular cloning, tissue distribution and expression analysis of a manganese superoxide dismutase in blunt snout bream Megalobrama amblycephala. Sun, Shengming,Zhu, Jian,Li, Bing,Ge, Xianping,Jiang, Xiaojun,Ge, Xianping.

[12]Nitrite-induced hepatotoxicity in Bluntsnout bream (Megalobrama amblycephala): The mechanistic insight from transcriptome to physiology analysis. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Yu, Na.

[13]Chemokine receptor CXCR3 in turbot (Scophthalmus maximus): cloning, characterization and its responses to lipopolysaccharide. Chen, Yadong,Zhou, Shuhong,Jiang, Zhiqiang,Wang, Xiuli,Liu, Yang,Chen, Yadong.

[14]Effect of crowding stress on the immune response in turbot (Scophthalmus maximus) vaccinated with attenuated Edwardsiella tarda. Huo, Huanhuan,Jia, Rui,Lei, Jilin,Liu, Baoliang,Huo, Huanhuan,Yin, Shuting,Jia, Rui,Huang, Bin,Lei, Jilin,Liu, Baoliang.

[15]Isolation and characterization of microsatellite markers for turbot (Scophthalmus maximus) by screening SSR-enriched library. Gu, Ying,Zhao, Yingying,Lu, Cuiyun,Sun, Xiaowen,Gu, Ying,Zhao, Yingying,Liu, Yongxin.

[16]Molecular cloning and expression analysis of a hepcidin antimicrobial peptide gene from turbot (Scophthalmus maximus). Chen, Song-Lin,Li, Wei,Meng, Liang,Sha, Zhen-Xia,Wang, Zhi-Jian,Ren, Guo-Cheng. 2007

[17]Effect of size-fractionated fish protein hydrolysate on growth and feed utilization of turbot (Scophthalmus maximus L.). Zheng, Keke,Liang, Mengqing,Yao, Hongbo,Wang, Jialin,Chang, Qing. 2013

[18]Morphological comparison between a selected fast-growing strain and the common cultured strain of turbot Scophthalmus maximus. Liang Xingming,Ma Aijun,Wang Xin'an,Li Juan,Huang Zhihui,Shang Xiaomei. 2012

[19]Molecular identification and expression analysis of the natural killer cell enhancing factor (NKEF) gene from turbot (Scophthalmus maximus). Chen, Ying,Zhang, Yu-Xi,Fan, Ting-Jun,Meng, Liang,Ren, Guo-cheng,Chen, Song-Lin. 2006

[20]Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Chen, SL,Ren, GC,Sha, ZX,Hong, YH. 2005

作者其他论文 更多>>