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Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus)

文献类型: 外文期刊

作者: Jia, Rui 1 ; Han, Cen 3 ; Lei, Ji-Lin 2 ; Liu, Bao-Liang 2 ; Huang, Bin 2 ; Huo, Huan-Huan 1 ; Yin, Shu-Ting 2 ;

作者机构: 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China

3.Dalian Ocean Univ, Coll Fisheries & Life Sci, Dalian 116023, Peoples R China

关键词: Nitrite;Oxidative stress;Apoptosis;Gills;Scophthalmus maximus

期刊名称:AQUATIC TOXICOLOGY ( 影响因子:4.964; 五年影响因子:5.071 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Nitrite (NO2-) is commonly present as contaminant in aquatic environment and toxic to aquatic organisms. In the present study, we investigated the effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). Fish were exposed to various concentrations of nitrite (0, 0.02, 0.08, 0.4 and 0.8 mM) for 96 h. Fish blood and gills were collected to assay haematological parameters, oxidative stress and expression of genes after 0, 24, 48 and 96 h of exposure. In blood, the data showed that the levels of methemoglobin (MetHb), triglyceride (TG), potassium (K+), cortisol, heat shock protein 70 (HSP70) and glucose significantly increased in treatments with higher concentrations of nitrite (0.4 and/or 0.8 mM) after 48 and 96 h, while the levels of haemoglobin (Hb) and sodium (Na+) significantly decreased in these treatments. In gills, nitrite (0.4 and/or 0.8 mM) apparently reduced the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione (GSH), increased the formation of malondialdehyde (MDA), up-regulated the mRNA levels of c-jun amino-terminal kinase (JUK1), p53, caspase-3, caspase-7 and caspase-9 after 48 and 96 h of exposure. The results suggested caspase-dependent and JUK signaling pathways played important roles in nitrite-induced apoptosis in fish. Further, this study provides new insights into how nitrite affects the physiological responses and apoptosis in a marine fish. (C) 2015 Elsevier B.V. All rights reserved.

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