您好,欢迎访问广东省农业科学院 机构知识库!

Comparative Transcriptome Analysis Reveals Differential Transcription in Heat-susceptible and Heat-tolerant Pepper (Capsicum annum L.) Cultivars under Heat Stress

文献类型: 外文期刊

作者: Li, Tao 1 ; Xu, Xiaowan 1 ; Li, Ying 1 ; Wang, Hengming 1 ; Li, Zhiliang 1 ; Li, Zhenxing 1 ;

作者机构: 1.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Key Lab New Technol Res Vegetables, Guangzhou 510640, Guangdong, Peoples R China

关键词: Capsicum annuum L;Gene expression;Heat stress;RNA-Seq;Transcriptome

期刊名称:JOURNAL OF PLANT BIOLOGY ( 影响因子:2.434; 五年影响因子:2.455 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Heat stress is an important agricultural problem around the world. In pepper (Capsicum annum L.), heat stress seriously affects pollination and yield. However, to date, the molecular basis of heat stress has not been extensively studied. Using the HiSeq TM 2000 sequencing platform, the seedling transcriptome of heat-susceptible C. annuum 'S590'(CaS) and heat-tolerant 'R597' (CaR) under the heat stress was examined. Over five million clean reads were generated from each library, each corresponding to a coverage of > 250,000 nt. About 73% of the reads were mapped to the pepper genome, and 3,799 and 4,010 differentially expressed genes (DEGs) were identified in 'R597' (CaR) and 'S590'(CaS), respectively. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses determined that the identified DEGs were involved in heat shock protein, heat shock transcription factors, hormone, as well as calcium and kinase signaling. Further validation identified 35 genes that were involved in stress response, and that most of the heat shock proteins were upregulated in two genotypes, and highly expressed in susceptible S590 than in tolerant cultivar R597; the transcription factors and hormone signaling genes showed higher levels of expression in the heat-tolerant cultivar R597 than that observed in the heat-susceptible S590. These findings facilitate in better understanding of the molecular mechanism underlying heat stress in different pepper genotypes.

  • 相关文献

[1]De novo Transcriptome Assembly of Chinese Kale and Global Expression Analysis of Genes Involved in Glucosinolate Metabolism in Multiple Tissue. Wu, Shuanghua,Lei, Jianjun,Chen, Guoju,Cao, Bihao,Chen, Changming,Chen, Hancai. 2017

[2]Transcriptome comparison in the pituitary-adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure. Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Fang, Meixia,Xing, Huijie.

[3]基于RNA-seq筛选影响猪肉品质的关键候选基因. 肖明飞,申露露,何逸懿,易宏波,唐青松,李莉,洪兴,李大江,徐娥. 2022

[4]辣椒杂交种与亲本苗期叶片基因差异表达分析. 徐小万,李颖,王恒明,李涛,何水林,官德义. 2016

[5]玫烟色棒束孢诱导的小菜蛾免疫响应表达谱分析. 雷妍圆,何余容,谢梅琼,吕利华,顾家睿. 2016

[6]Genome-Wide Identification and Expression Profile of Dof Transcription Factor Gene Family in Pepper (Capsicum annuum L.). Wu, Zhiming,Bang, Guansheng,Cheng, Jiaowen,Cui, Junjie,Hu, Kailin,Xu, Xiaowan,Luo, Xirong,Chen, Xiaocui,Tang, Xiangqun,Qin, Cheng,Qin, Cheng. 2016

[7]Characterization of HSP70 and its expression in tissue: correlation with physiological and immune indices in goose (Anser cygnoides) serum. Zhang, W. W.,Zhang, X. Q.,Kong, L. N.,Luo, Q. B.,Zhang, W. W.,Xiao, X.,Gan, J. K.,Zhang, X. Q.,Kong, L. N.,Luo, Q. B.. 2015

[8]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[9]Effects of heat stress on gene expression in eggplant (Solanum melongema L.) seedlings. Li, Yanyan,Li, Zhiliang,Li, Zhenxing,Luo, Shaobo,Sun, Baojuan,Li, Yanyan. 2011

[10]Heat stress impairs the nutritional metabolism and reduces the productivity of egg-laying ducks. Ma, Xianyong,Lin, Yingcai,Zhang, Hanxing,Chen, Wei,Wang, Shang,Ruan, Dong,Jiang, Zongyong,Ma, Xianyong,Lin, Yingcai,Zhang, Hanxing,Chen, Wei,Wang, Shang,Ruan, Dong,Jiang, Zongyong,Ma, Xianyong,Lin, Yingcai,Zhang, Hanxing,Chen, Wei,Wang, Shang,Ruan, Dong,Jiang, Zongyong. 2014

[11]Integrating Small RNA Sequencing with QTL Mapping for Identification of miRNAs and Their Target Genes Associated with Heat Tolerance at the Flowering Stage in Rice. Liu, Qing,Yang, Tifeng,Zhang, Shaohong,Mao, Xingxue,Zhao, Junliang,Wang, Xiaofei,Dong, Jingfang,Liu, Bin,Liu, Qing,Yang, Tifeng,Zhang, Shaohong,Mao, Xingxue,Zhao, Junliang,Wang, Xiaofei,Dong, Jingfang,Liu, Bin,Yu, Ting. 2017

[12]Strong, sudden cooling alleviates the inflammatory responses in heat-stressed dairy cows based on iTRAQ proteomic analysis. Cheng, Jianbo,Fan, Caiyun,Cheng, Jianbo,Min, Li,Zheng, Nan,Zhao, Shengguo,Zhang, Yangdong,Wang, Jiaqi,Min, Li. 2018

[13]Genome-wide analysis of gene expression profiles during early ear development of sweet corn under heat stress. Li, Yuliang,Hu, Jianguang,Liu, Jianhua,Suo, Haicui,Yu, Yongtao,Han, Fuguang.

[14]Overexpression of mitochondrial uncoupling protein conferred resistance to heat stress and Botrytis cinerea infection in tomato. Chen, Shuangchen,Liu, Airong,Zhang, Shaojie,Li, Cong,Chang, Rui,Lin, Xiaomin,Ahammed, Golam Jalal,Liu, Dilin.

[15]The association of SNPs in Hsp90 beta gene 5 ' flanking region with thermo tolerance traits and tissue mRNA expression in two chicken breeds. Chen, Zhuo-Yu,Gan, Jian-Kang,Xiao, Xiong,Jiang, Li-Yan,Zhang, Xi-Quan,Luo, Qing-Bin,Chen, Zhuo-Yu,Gan, Jian-Kang,Xiao, Xiong,Jiang, Li-Yan,Zhang, Xi-Quan,Luo, Qing-Bin.

[16]RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Xing, Dongxu,Jiang, Liang,Xia, Qingyou,Xing, Dongxu,Yang, Qiong,Li, Qingrong,Xiao, Yang,Ye, Mingqiang. 2017

[17]Transcriptome profiling of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f. sp cubense tropical race 4. Li, Chun-yu,Jin, Yan,Kuang, Rui-bin,Yang, Qiao-song,Sheng, Ou,Wei, Yue-rong,Hu, Chun-hua,Dong, Tao,Yi, Gan-jun,Li, Chun-yu,Jin, Yan,Kuang, Rui-bin,Yang, Qiao-song,Sheng, Ou,Wei, Yue-rong,Hu, Chun-hua,Dong, Tao,Yi, Gan-jun,Deng, Gui-ming,Yang, Jing,Zuo, Cun-wu,Lv, Zhi-cheng,Viljoen, Altus. 2012

[18]GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens. Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Lin, Wencheng,Chen, Weiguo,Chen, Feng,Xie, Qingmei,Zhang, Huanmin. 2016

[19]Comparative transcriptome analysis of sweet corn seedlings under low-temperature stress. Mao, Jihua,Yu, Yongtao,Yang, Jing,Li, Gaoke,Li, Chunyan,Qi, Xitao,Wen, Tianxiang,Hu, Jianguang. 2017

[20]Transcriptome sequencing reveals genetic mechanisms underlying the transition between the laying and brooding phases and gene expression changes associated with divergent reproductive phenotypes in chickens. Shen, Xu,Shen, Xu,Shen, Xu,Xu, Haipin,Nie, Qinghua,Zhang, Xiquan,Shen, Xu,Xu, Haipin,Nie, Qinghua,Zhang, Xiquan,Shen, Xu,Bai, Xue,Xu, Jin,Lu, Xuemei,Zhou, Min.

作者其他论文 更多>>