文献类型： 外文期刊

作者： Li, B. -Q. ¹ ; Feng, C. -H. ¹ ; Hu, L. -Y. ¹ ; Wang, M. -R. ¹ ; Wang, Q. -C. ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China

2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou, Peoples R China

关键词： Apple;ASGV;ASPV;cryopreservation;shoot tip culture;shoot tips

期刊名称：ANNALS OF APPLIED BIOLOGY （ 影响因子：2.75； 五年影响因子：2.942 ）

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收录情况： SCI

摘要： This study attempted to eradicate Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) from virus-infected in vitro shoots of apple rootstocks 'M9' and 'M26' using shoot tip culture and cryopreservation. In shoot tip culture, shoot tips (0.2 mm in length) containing two leaf primordia failed to show shoot regrowth. Although shoot regrowth rate was the highest in the largest shoot tips (1.0 mm in length) containing four leaf primordia, none of the regenerated shoots was virus-free. Shoot tips (0.5 mm in length) containing two and three leaf primordia produced 100% and 10% of ASPV-free shoots, respectively, while those (1.0 mm) containing four leaf primordia were not able to eradicate ASPV. ASGV could not be eradicated by shoot tip culture, regardless of the size of the shoot tips tested. In cryopreservation, shoot tips (0.5 mm in length) containing two leaf primordia did not resume shoot growth. Although 1.0-mm and 1.5-mm shoot tips gave similarly high ASPV-free frequencies, the latter had much higher shoot regrowth rate than the former. Very similar results of shoot regrowth and virus eradication by shoot tip culture and cryopreservation were observed in both 'M9' and 'M26'. Histological observations showed that only cells in upper part of apical dome and in leaf primordia 1-3 survived, while other cells were damaged or killed, in shoot tips following cryopreservation. Virus immunolocalization found ASPV was not detected in upper part of apical dome and leaf primordia 1 and 2, but was present in lower part of apical dome, and in leaf primordium 4 and more developed tissues in all samples tested. ASPV was also detected in leaf primordium 3 in about 16.7% and 13.3% samples tested in 'M9' and 'M26'. ASGV was observed in apical dome and leaf primordia 1-6, leaving only a few top layers of cells in apical dome free of the virus. Different abilities of ASPV and ASGV to invade leaf petioles and shoot tips were also noted.