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Effects of water management practices on residue decomposition and degradation of Cry1Ac protein from crop-wild Bt rice hybrids and parental lines during winter fallow season

文献类型： 外文期刊

作者： Xiao, Manqiu 1 ; Dong, Shanshan 1 ; Li, Zhaolei 1 ; Tang, Xu 2 ; Chen, Yi 2 ; Yang, Shengmao 2 ; Wu, Chunyan 3 ; Ouyang, 1 ;

作者机构： 1.Fudan Univ, Minist Educ, Inst Biodivers Sci, Key Lab Biodivers Sci & Ecol Engn, Shanghai 200433, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Environm Resources Soils & Fertilizers, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhej

关键词： Bt rice;Ecological risk;Crop-wild hybrid;Cry1Ac protein;Litter decomposition;Water management

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rice is the staple diet of over half of the world's population and Bacillus thuringiensis (Bt) rice expressing insecticidal Cry proteins is ready for deployment. An assessment of the potential impact of Bt rice on the soil ecosystem under varied field management practices is urgently required. We used litter bags to assess the residue (leaves, stems and roots) decomposition dynamics of two transgenic rice lines (Kefeng6 and Kefeng8) containing stacked genes from Bt and sck (a modified CpTI gene encoding a cowpea trypsin inhibitor) (Bt/CpTI), a non-transgenic rice near-isoline (Minghui86), wild rice (Oryza rufipogon) and crop-wild Bt rice hybrid under contrasting conditions (drainage or continuous flooding) in the field. No significant difference was detected in the remaining mass, total C and total N among cultivars under aerobic conditions, whereas significant differences in the remaining mass and total C were detected between Kefeng6 and Kefeng8 and Minghui86 under the flooded condition. A higher decomposition rate constant (km) was measured under the flooded condition compared with the aerobic condition for leaf residues, whereas the reverse was observed for root residues. The enzyme-linked immunosorbent assay (ELISA), which was used to monitor the changes in the Cry1Ac protein in Bt rice residues, indicated that (1) the degradation of the Cry1Ac protein under both conditions best fit first-order kinetics, and the predicted DT50 (50% degradation time) of the Cry1Ac protein ranged from 3.6 to 32.5 days; (2) the Cry1Ac protein in the residue degraded relatively faster under aerobic conditions; and (3) by the end of the study (similar to 154 days), the protein was present at a low concentration in the remaining residues under both conditions. The degradation rate constant was negatively correlated with the initial carbon content and positively correlated with the initial Cry1Ac protein concentration, but it was only correlated with the mass decomposition rate constants under the flooded condition. No Cry1Ac protein was detected in the soils surrounding the buried residue. Our results did not reveal any evidence that the stacked genes (Bt/CpTI) or the presence of the Cry1Ac protein influenced the decomposition dynamics of the rice residues. Furthermore, our results suggested that field drainage after residue incorporation would promote Cry1Ac protein degradation. (C) 2015 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Litterbag decomposition of residues from Bacillus thuringiensis (Bt) rice hybrids and the parental lines under multiple field conditions. Xiao, Manqiu,Fang, Changming,Dong, Shanshan,Luo, Fuhua,Song, Zhiping,Tang, Xu,Chen, Yi,Yang, Shengmao,Wang, Feng,Su, Jun. 2014

[2]OCCURRENCE OF WEEDS IN WET-SEEDED RICE FIELDS AND APPLICATION OF NOMINEE (BISPYRIBAC-SODIUM) IN ZHEJIANNG, CHINA. Q. Wang,X. P. Zhao,C. X. Wu,F. Dai,L. Q. Wu,H. Xu,M. S. Li,J. H. He. 2001

[3]Effects of two bt rice lines T2A-1 and T1C-19 on the ecological fitness and detoxification enzymes of nilaparvata lugens (hemiptera: Delphacidae) from different populations. Yang, Yajun,He, Jingjing,Dong, Biqin,Xu, Hongxing,Zheng, Xusong,Lu, Zhongxian,He, Jingjing,Fu, Qiang,Lin, Yongjun,Lin, Yongjun.

[4]Transgenic cry1C or cry2A rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Liu, Yu-E,Han, Nai-Shun,Hu, Cui,Ye, Gong-Yin,Lu, Zeng-Bin,Liu, Yu-E,Han, Nai-Shun,Hu, Cui,Ye, Gong-Yin,Tian, Jun-Ce,Peng, Yu-Fa,Guo, Yu-Yuan.

[5]Cry1Ab rice does not impact biological characters and functional response of Cyrtorhinus lividipennis preying on Nilaparvata lugens eggs. Chen Yang,Lai Feng-xiang,Sun Yan-qun,Hong Li-ying,Zhang Zhi-tao,Fu Qiang,Tian Jun-ce. 2015

[6]Impacts of Bt Rice Expressing Cry1C or Cry2A Protein on the Performance of Nontarget Leafhopper, Nephotettix cincticeps (Hemiptera: Cicadellidae), Under Laboratory and Field Conditions. Wang, W.,Hu, C.,Ye, G. Y.,Lu, Z. B.,Wang, W.,Hu, C.,Ye, G. Y.,Tian, J. C.,Xu, H. X.,Guo, Y. Y.,Peng, Y. F.. 2014

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