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Loop-mediated isothermal amplification assay for sensitive and rapid detection of Phytophthora capsici

文献类型： 外文期刊

作者： Dong, Zhongmei 1 ; Liu, Peiqing 1 ; Li, Benjin 1 ; Chen, Guoliang 1 ; Weng, Qiyong 1 ; Chen, Qinghe 1 ;

作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fuzhou 350003, Peoples R China

2.Fujian Agr & Forestry Univ, Fujian Taiwan Joint Innovat Ctr Ecol Control Crop, Fuzhou 350002, Peoples R China

关键词： calcein;LAMP;molecular detection;pathogen detection;Phytophthora capsici

期刊名称：CANADIAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.442； 五年影响因子：1.993 ）

ISSN：

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收录情况： SCI

摘要： Phytophthora capsici is an oomycete plant pathogen that infects peppers, tomatoes, and other agronomic and ornamental crops of the Solanaceae and Cucurbitaceae families worldwide. Early and accurate detection of P. capsici is essential for controlling this disease in the field. The internal transcribed spacer (ITS) regions of nuclear-encoded ribosomal DNA genes are widely used to detect Phytophthora nicotianae, Pythium helicoides and Peronospora variabilis. In this study, a novel and highly sensitive loop-mediated isothermal amplification (LAMP) assay was developed for the specific detection of P. capsici using calcein as a fluorescent indicator. Four LAMP primers were designed based on the ITS sequence of P. capsici. A total of 23 isolates of P. capsici from geographically distinct counties in China yielded positive results in the LAMP assay. No cross-reaction was observed with other oomycetes or fungal pathogens. The detection limit of P. capsici by LAMP was 100fg genomic DNA per 25L reaction. The LAMP assay developed in this study is simple, fast, sensitive and specific, and can be used in the field to detect P. capsici in infected plant tissue.

  • 相关文献

[1]Development of a specific PCR assay for the rapid and sensitive detection of Phytophthora capsici. Lan, C. Z.,Liu, P. Q.,Li, B. J.,Chen, Q. H.,Weng, Q. Y.. 2013

[2]南洋臀纹粉蚧生物学特性及LAMP检测. 黄鹏,张杰,姚锦爱,蓝炎阳,余德亿. 2023

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[4]Development and evaluation of specific PCR and LAMP assays for the rapid detection of Phytophthora melonis. Chen, Qinghe,Li, Benjin,Liu, Peiqing,Lan, Chengzhong,Zhan, Zhixiong,Weng, Qiyong.

[5]Activity of the novel fungicide oxathiapiprolin against plant-pathogenic oomycetes. Miao, Jianqiang,Dong, Xue,Lin, Dong,Wang, Qiushi,Liu, Pengfei,Liu, Xili,Chen, Furu,Du, Yixin.

[6]The L-type Ca2+ Channel Blocker Nifedipine Inhibits Mycelial Growth, Sporulation, and Virulence of Phytophthora capsici. Liu, Peiqing,Gong, Jie,Ding, Xueling,Jiang, Yue,Chen, Guoliang,Li, Benjin,Weng, Qiyong,Chen, Qinghe. 2016

[7]Development and evaluation of ITS- and aflP-based LAMP assays for rapid detection of Aspergillus flavus in food samples. Liu, Peiqing,Li, Benjin,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe.

[8]Specific and Sensitive Detection of Phytophthora nicotianae by Nested PCR and Loop-mediated Isothermal Amplification Assays. Li, Benjin,Liu, Peiqing,Xie, Shiyong,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe,Chen, Qinghe.

[9]Evaluation of Different PCR-Based Assays and LAMP Method for Rapid Detection of Phytophthora infestans by Targeting the Ypt1 Gene. Khan, Mehran,Jiang, Yue,Weng, Qiyong,Chen, Qinghe,Khan, Mehran,Li, Benjin,Weng, Qiyong,Chen, Qinghe. 2017

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