PlAtg8-mediated autophagy regulates vegetative growth, sporangial cleavage, and pathogenesis in Peronophythora litchii
文献类型： 外文期刊
作者： Yu, Ge 1 ; Li, Wenqiang 3 ; Yang, Chengdong 1 ; Zhang, Xue 1 ; Luo, Manfei 1 ; Chen, Taixu 1 ; Wang, Xuejian 1 ; Wang, Rongbo 3 ; Chen, Qinghe 1 ;
作者机构： 1.Hainan Univ, Sch Trop Agr & Forestry, Sanya Inst Breeding & Multiplicat, Sch Breeding & Multiplicat, Sanya, Peoples R China
2.Hainan Univ, Minist Educ, Key Lab Green Prevent & Control Trop Dis & Pests, Haikou, Peoples R China
3.Fujian Acad Agr Sci, Inst Plant Protect, Fujian Key Lab Monitoring & Integrated Management, Fuzhou, Peoples R China
关键词： PlAtg8; sporangial cleavage; autophagosome formation; pathogenicity; Peronophythora litchii
期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）
ISSN： 2165-0497
年卷期： 2024 年 12 卷 1 期
页码：
收录情况： SCI
摘要： Autophagy is a conserved cellular metabolic process in eukaryotes that transports damaged or redundant intracellular organelles and proteins to lysosomes or vacuoles for degradation and recycling. Atg8 is not only essential for autophagosome formation but also required for fungal development and pathogenicity, but its function is unknown in Peronophythora litchii. In this study, PlAtg8, a yeast Atg8 homolog, is deleted by the CRISPR/Cas9 system coupled with PEG-mediated protoplast transformation in P. litchii. Compared with the wild-type strain, the absence of PlAtg8 affects autophagosome formation. The colony growth rate, number of sporangia, and zoospore release rate are significantly decreased, and the oospores are undetected in Delta Platg8. The pathogenicity test shows that the lesion areas on litchi leaves and fruits caused by Delta Platg8 are significantly attenuated compared with the wild type, indicating that PlAtg8 is also required for the pathogenicity of P. litchii. Moreover, the cleavage rate of sporangia in Delta Platg8 is significantly decreased compared to the wild type by FM4-64 staining, which is probably the main reason for the lower zoospores release rate in Delta Platg8. These results suggest that PlAtg8 not only regulates autophagy but also participates in the growth and development of P. litchii.
- 相关文献
作者其他论文 更多>>
-
Dimethyl trisulfide (DMTS) fumigation enhances fruit quality and reduces postharvest incidence of bacterial canker in tomato
作者：Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing;Zhang, Qianrong;Liu, Peiqing
关键词：Bacterial canker of tomato; DMTS; Postharvest; Antioxidants; Ethylene biosynthesis
-
Rapid Visual Detection of Peronophythora litchii on Lychees Using Recombinase Polymerase Amplification Combined with Lateral Flow Assay Based on the Unique Target Gene Pl_101565
作者：Wang, Rongbo;Li, Benjin;Shi, Mingyue;Zhao, Yumei;Lin, Jinlong;Liu, Peiqing;Chen, Qinghe
关键词：Peronophythora litchii; recombinase polymerase amplification; lateral flow; lychee
-
A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector
作者：Cao, Peng;Shi, Haotian;Zhang, Shuangxi;An, Yuyan;Zhang, Meixiang;Shi, Haotian;Chen, Jialan;Wang, Rongbo;Liu, Peiqing;Zhu, Yingfang
关键词：TMV-GFP; effector; virulence; avirulence; functional screening; technical advance
-
Bacillus velezensis stimulated the absorption of iodine to improve antioxidants and delay post-harvest senescence in microgreens
作者：Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing;Jin, Ting;Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing
关键词：Microgreens; Bacillus velezensis; Iodine; Antioxidants; Senescence
-
Identification and expression profiling of GAPDH family genes involved in response to Sclerotinia sclerotiorum infection and phytohormones in Brassica napus
作者：Xu, Jing;Zhuang, Wei;Zhang, Yang;Lin, Jianxin;Zhan, Penglin;Chen, Shanhu;Lu, Heding;Liao, Changjian;Xu, Jing;Wang, Airong;Wang, Rongbo;Zhang, Xiong
关键词：Brassica napus; GAPDH; gene family; Sclerotinia sclerotiorum; subcellular localization; nuclear translocation
-
Genetic Diversity of Ralstonia solanacearum Causing Tobacco Bacterial Wilt in Fujian Province and Identification of Biocontrol Streptomyces sp.
作者：Wang, Rongbo;Li, Benjin;Cai, Songling;Shi, Mingyue;Liu, Peiqing;Ding, Yingfu;Lin, Wei;Jin, Ting
关键词：antimicrobial activity; bacterial wilt; phylotype; sequevar; Streptomyces
-
Efficacy of 2,4-Di-tert-butylphenol in Reducing Ralstonia solanacearum Virulence: Insights into the Underlying Mechanisms
作者：Yang, Le;Wang, Rongbo;Li, Benjing;Weng, Qiyong;Liu, Peiqing;Lin, Wei;Liu, Peiqing;Jin, Ting;Zhang, Meixiang
关键词：
