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Identification of a bacterium isolated from the diseased brown planthopper and determination of its insecticidal activity

文献类型: 外文期刊

作者: Niu, Hongtao 1 ; Liu, Baosheng 1 ; Li, Yongteng 1 ; Guo, Huifang 1 ;

作者机构: 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Jiangsu, Peoples R China

关键词: Nilaparvata lugens;Serratia;bacteria;rice;biocontrol

期刊名称:BIOCONTROL SCIENCE AND TECHNOLOGY ( 影响因子:1.665; 五年影响因子:1.616 )

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收录情况: SCI

摘要: The brown planthopper, Nilaparvata lugens, is one of the most harmful insect pests of rice crops in Asian countries. To find an effective biological control agent against this pest, we investigated the bacterial flora of field N. lugens collected from Jiangsu Province, China, in 2012 and tested its insecticidal activity. A novel bacterium strain, S-JS1, was isolated from N. lugens nymphs and adults and showed a high level of insecticidal activity. Based on its phenotypic, physiological and biochemical properties, and its 16S rRNA gene phylogeny, the isolate was assigned to Serratia marcescens; the name S. marcescens S-JS1 is proposed. The pathogenicity of S-JS1 against the third-instar nymphs, and the macropterous and the brachypterous adults of N. lugens were compared. The median lethal concentration (LC50) values of S-JS1 against the brachypterous adult were the lowest (LC50, 1.53 x 10(8) colony forming units (cfu)/ml), followed against the macropterous adult (LC50, 1.65 x 10(9) cfu/ml) and third-instar nymphs (LC50, 1.86 x 10(9) cfu/ml) at 5 days post-infection. The median lethal time values of 8 x 10(8) cfu/ml S. marcescens S-JS1 against the brachypterous adult, macropterous adult, and third-instar nymph were 4.5, 5.5, and 5.7 days, respectively. These results indicate that the S-JS1 isolate appears to be a promising S. marcescens strain with strong biocontrol potential against N. lugens.

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