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Testes transcriptome profiles of the anadromous fish Coilia nasus during the onset of spermatogenesis

文献类型： 外文期刊

作者： Zhou, Yan-Feng 1 ; Duan, Jin-Rong 1 ; Liu, Kai 1 ; Xu, Dong-Po 1 ; Zhang, Min-Ying 1 ; Fang, Di-An 1 ; Xu, Pao 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214128, Peoples R China

2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214128, Peoples R China

关键词： Illumina sequencing;Transcriptome;Coilia nasus;Spermatogenesis

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

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收录情况： SCI

摘要： RNA-Seq technology has been widely applied to transcriptomics, genomics and functional gene study. Here, we performed de novo transcriptome sequencing to produce 23,842,172 clean reads representing a total of 4,815,798,404 (4.8 Gb) nucleotides from comprehensive transcript dataset for testis of Coilia nasus. Over 20 million Illumina reads were assembled into 194,636 unigenes, and 42,642 annotated genes were predicted by Blastx and ESTScan, respectively. Applying Blast analysis and functional annotation (e.g., GO, COG, SwissProt and I(EGG) using the assembled gene models from catalogs of other species, we have sampled an extensive and diverse expressed gene catalog for C. nasus representing a large proportion of the genes involved in the onset of spermatogenesis. The results will provide a general clue to the potential spermatogenesis molecular mechanisms for this species. (c) 2015 Elsevier B.V. All rights reserved.

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