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A Study of 2,3,7,8-Tetrachlorodibenzo-p-dioxin Induced Liver Injury in Jian Carp (Cyprinus carpio var. Jian) Using Precision-Cut Liver Slices

文献类型： 外文期刊

作者： Du, Jin-Liang 1 ; Cao, Li-Ping 1 ; Liu, Ying-Juan 3 ; Jia, Rui 3 ; Yin, Guo-Jun 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Minist Agr, Key Lab Freshwater Fisheries & Germplasm Resource, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Int Joint Res Lab Fish Immunopharmacol, Wuxi 214081, Peoples R China

3.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

4.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R

关键词： Jian Carp;2;3;7;8-Tetrachlorodibenzo-p-dioxin;AhR2;ARNT2;CYP1A

期刊名称：BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY （ 影响因子：2.151； 五年影响因子：2.129 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The aim of this study was to establish a model for the study of liver injury induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in Jian carp using precision-cut liver slices (PCLS). PCLS were treated with TCDD at concentrations of 0, 0.05, 0.1, 0.3, and 0.6 mu g/L for 6 h, followed by collection of the culture supernatant and PCLS for analysis. Several biochemical indices were analyzed, including glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA). Expression of mRNA was also estimated for cytochrome P4501A (CYP1A), aryl hydrocarbon receptor2 (AhR2), and aryl hydrocarbon receptor nuclear translocator2 (ARNT2). Results showed that some significant effects (p < 0.05) in MDA, GSH-Px and PCLS viability were observed at a TCDD concentration as low as 0.05 mu g/L, and the observed effects increased with exposure concentration. Following exposure to TCDD for 6 h at a concentration of 0.3 mu g/L, significant increases (p < 0.01) in the content of GPT, GOT, MDA, and LDH were observed, while SOD activity, GSH-Px activity, and PCLS viability were decreased (p < 0.01 or p < 0.05). Exposure to 0.3 mu g/L TCDD also resulted in increased expression of mRNA for CYP1A, AhR2, and ARNT2. Overall, these results provide evidence of TCDD-induced liver injury and oxidative stress in Jian carp. These results also support the use of PCLS as an in vitro model for the evaluation of hepatotoxicity in Jian carp.

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