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Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum

文献类型： 外文期刊

作者： Zhang, Leigang 1 ; Li, Baicun 1 ; Zhang, Yu 1 ; Jia, Xiaojing 1 ; Zhou, Mingguo 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Nanjing Agr Univ, State & Local Joint Engn Res Ctr Green Pesticide, Nanjing 210095, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Jiangsu, Peoples

关键词： DON;FgHXK1;Fusarium graminearum;hexokinase;MBC resistance

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：5.663； 五年影响因子：5.626 ）

ISSN：

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收录情况： SCI

摘要： Fusarium graminearum, the causal agent of Fusarium head blight, is a common pathogen on small grain cereals worldwide and produces various trichothecenes [deoxynivalenol (DON) is predominant] during infection. A previous study has revealed that DON production is positively correlated with the occurrence of carbendazim (MBC) resistance. Here, we identified and characterized two putative genes encoding hexokinase in F. graminearum (FgHXK1 and FgHXK2), which is a rate-limiting enzyme in DON biosynthesis. The expression level of hexokinase genes and the production of pyruvate, which is the precursor of DON, were up-regulated in the MBC-resistant strain, indicating that hexokinase genes might be involved in increased DON production. Phylogenetic and comparative analyses indicated that FgHXK1 was the predominant hexokinase gene. Gene disruption showed that Delta FgHXK1 severely affected DON production, indicating that FgHXK1 played a role in the regulation of DON biosynthesis. Morphological characterization showed that Delta FgHXK1 led to inhibited vegetative growth and conidiation. Sensitivity tests to MBC and various stresses indicated that both Delta FgHXK1 and Delta FgHXK2 mutants showed no significant difference from parental strains. Pathogencity assays showed that Delta FgHXK1 mutants lost virulence on wheat head and corn stigma; however, they showed no change in sexual reproduction. The FgHXK1-overexpressing transformants were obtained subsequently. Their pyruvate and DON production was confirmed to be increased, indicating that FgHXK1 positively regulated DON biosynthesis. Although additional defects appeared in overexpression mutants, MBC sensitivity showed no change. All of the results indicated that the transcriptional level of FgHXK1 regulated DON biosynthesis, but showed no direct relationship with MBC resistance.

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[1]小麦赤霉病和DON毒素研究进展. 樊平声. 2010

[2]Evaluation of tebuconazole for the management of Fusarium head blight in China. Sun, H. -Y.,Zhu, Y. -F,Liu, Y. -Y.,Deng, Y. -Y.,Li, W.,Zhang, A. -X.,Chen, H. -G..

[3]Change of Defensive-related Enzyme in Wheat Crown Rot Seedlings Infected by Fusarium graminearum. Zhang, P.,Zhou, M. P.,Zhang, X.,Huo, Y.,Ma, H. X.. 2013

[4]Simultaneous determination of deoxynivalenol, and 15-and 3-acetyldeoxynivalenol in cereals by HPLC-UV detection. Yang, D.,Geng, Z. M.,Yao, J. B.,Zhang, X.,Zhang, P. P.,Ma, H. X.. 2013

[5]Enzyme-Linked Immunosorbent-Assay for Deoxynivalenol (DON). Ji, Fang,Li, Hua,Xu, Jianhong,Shi, Jianrong. 2011

[6]Fusarium graminearum growth inhibition due to glucose starvation caused by osthol. Shi, Zhiqi,Shen, Shouguo,Zhou, Wei,Wang, Fei,Fan, Yongjian. 2008

[7]Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China. Qiu, Jianbo,Shi, Jianrong. 2014

[8]Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Li, Zhao,Zhang, Zengyan,Du, Lipu,Xu, Huijun,Xin, Zhiyong,Li, Zhao,Zhou, Miaoping,Ren, Lijuan,Zhang, Boqiao.

[9]Involvement of threonine deaminase FgIlv1 in isoleucine biosynthesis and full virulence in Fusarium graminearum. Liu, Xin,Xu, Jianhong,Wang, Jian,Ji, Fang,Yin, Xianchao,Shi, Jianrong.

[10]Effects of plant height on type I and type II resistance to fusarium head blight in wheat. Yan, W.,Li, H. B.,Liu, C. J.,Yan, W.,Cai, S. B.,Ma, H. X.,Rebetzke, G. J.,Liu, C. J..

[11]Involvement of FgMad2 and FgBub1 in regulating fungal development and carbendazim resistance in Fusarium graminearum. Zhang, L. G.,Zhang, Y.,Li, B. C.,Jia, X. J.,Chen, C. J.,Zhou, M. G.,Zhang, L. G..

[12]Molecular characterization of the Fusarium graminearum species complex in Eastern China. Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong,Qiu, Jianbo,Xu, Jianhong,Shi, Jianrong.

[13]The Fungicidal Activity of Thymol against Fusarium graminearum via Inducing Lipid Peroxidation and Disrupting Ergosterol Biosynthesis. Gao, Tao,Chen, Jian,Shi, Zhiqi,Gao, Tao,Chen, Jian,Shi, Zhiqi,Gao, Tao,Chen, Jian,Shi, Zhiqi,Zhou, Hao,Zhou, Wei,Hu, Liangbin.

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