文献类型： 外文期刊

作者： Li, Keming ¹ ; Yang, Xiangbing ³ ; Xu, Guiying ¹ ; Cao, Yang ⁴ ; Lu, Baoqian ² ; Peng, Zhengqiang ² ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Banana & Plantain, Haikou 570102, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Haikou 570101, Peoples R China

3.Texas A&M Univ Syst, Texas A&M Agrilife Res & Extens Ctr, Weslaco, TX 78596 USA

4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

关键词： Asecodes hispinarum;Odorant-binding protein;Transcriptome sequencing;Tissue distribution

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

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收录情况： SCI

摘要： Asecodes hispinarum (Hymenoptera: Eulophidae) is an endoparasitoid and an efficient biological control agent which attacks larvae of Brontispa longissima, a serious insect pest of Palmae plants in China. Odorant binding proteins (OBPs) are believed to be important for transporting semiochemicals through the aqueous sensillar lymph to the olfactory receptor cells within the insect antennal sensilla. No previous study has reported on OBPs in A. hispinarum. In this study, we conducted the large-scale identification of OBP genes from the antennae of A. hispinarum by using transcriptome sequencing. Approximately 28.4 million total raw reads and about 27.3 million total clean reads were obtained, and then 46,363 unigenes were assembled. Of these unigenes, a total of 21,263 can be annotated in the NCBI non-redundant database. Among the annotated unigenes, 16,623 of them can be assigned to GO (Gene Ontology). Furthermore, we identified 8 putative OBP genes, and a phylogenetic tree analysis was performed to characterize the 8 OBP genes. In addition, the expression of the 8 OBP genes in different A. hispinarum body tissues was analyzed by real-time quantitative polymerase chain reaction (qRT-PCR). The results indicated that the 8 OBP genes were expressed accordingly to sexes and tissues, but all highly expressed in antennae. The finding of this study will lay the foundation for unraveling molecular mechanisms of A. hispinarum chemoperception. (C) 2015 The Authors. Published by Elsevier Inc.