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Effect of postharvest nitric oxide treatment on the proteome of peach fruit during ripening

文献类型: 外文期刊

作者: Kang, Ruoyi 1 ; Zhang, Li 2 ; Jiang, Li 1 ; Yu, Mingliang 3 ; Ma, Ruijuan 3 ; Yu, Zhifang 1 ;

作者机构: 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

2.Suzhou Vocat Univ, Coll Educ & Humanity, Suzhou 215104, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Hort, Nanjing 210014, Jiangsu, Peoples R China

关键词: Nitric oxide;Proteome;Peach fruit;Ethylene;Antioxidant enzymes;TCA cycle

期刊名称:POSTHARVEST BIOLOGY AND TECHNOLOGY ( 影响因子:5.537; 五年影响因子:5.821 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Nitric oxide (NO) is an important signalling molecule with diverse physiological functions in plants. This study investigated the protein profile changes of harvested peach fruit (Prunus persica L. cv. 'Xiahui NO.5') exposed to NO gas treatment. A total of 104 protein spots with abundance changes (>two-fold) in response to NO were observed, and the spots were further functionally classified into 7 categories, including energy and metabolism (30.77%), stress response and defence (25.00%), cell structure (8.65%), protein fate (8.65%), transport and transduction (6.73%), ripening and senescence (5.77%) and unclassified (13.46%). The effects of NO at the proteomic level in peach fruit are complicated and involve various biological processes. NO could induce an abundant increase of superoxide dismutase (SOD) and the ascorbate-glutathione cycle enzymes to promote the production of the complex 1-aminocyclopropane-1-carboxylic acid oxidase (ACO-NO-ACC), which affects the generation of ethylene. Also, NO could inhibit electron transport and oxygen consumption, enhance the tricarboxylic acid cycle (TCA) cycle and the glycolysis pathway, and NO could repress the loss of Ca2+ ions and other structural components that maintain the mechanical properties of cell structures. (C) 2015 Elsevier B.V. All rights reserved.

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