您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization and expression of cyp19a gene in the Chinese giant salamander Andrias davidianus

文献类型： 外文期刊

作者： Hu, Qiaomu 1 ; Xiao, Hanbing 1 ; Tian, HaiFeng 1 ; Meng, Yan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wudayuan First Rd 8, Wuhan 430223, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China

关键词： Andrias davidianus;p450 aromatase;Gene expression;Methylation

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We cloned the full length cyp19a of Chinese giant salamander Andrias davidianus, determined its distribution in tissues and developing gonads, and analyzed the CpG methylation pattern of the cyp19a promoter. The results revealed isoforms of 1706 bp (G arom) and 1698 bp (B arom) in length, differing in the 5' flanking region, both encoding 502 amino acids. The G arom gene was observed mainly in the ovary and kidney, with little in other investigated tissues, while B arom expression was high in the brain, ovary, testis, and pituitary, with low or undetected expression in other examined tissues. Total aromatase expression was high in the ovary; moderate in the kidney, brain, testis, and pituitary; and low in the remaining tissues. G arom expression was significantly higher in the ovary than in the testis and gradually decreased with maturation of the salamander. A single injection of methyltestosterone or letrozole resulted in ovarian G arom expression decreasing over a 12-96 h period. A 1366 bp sequence of the cyp19a promoter was cloned and shown to be conserved in selected species. CpG methylation level was negatively correlated with cyp19a expression in the examined tissues and developing ovaries. Five and three CpG methylation sites positively correlated with DNA methylation levels in tissues and developing ovary, suggesting that they play an important role in regulating cyp19a expression. The aromatase gene showed two isoforms with distinct expression patterns, and the promoter methylation level at specific CpG sites was associated with variation in expression profiles of tissues and developing ovaries. (C) 2015 Elsevier Inc All rights reserved.

  • 相关文献

[1]野生和养殖大鲵群体遗传多样性的微卫星分析. 孟彦,杨焱清,张燕,肖汉兵. 2008

[2]Comparative transcriptome reveal the potential adaptive evolutionary genes in Andrias davidianus. Hu, Qiaomu,Wang, Quanhe,Meng, Yan,Tian, Haifeng,Xiao, Hanbing,Wang, Quanhe. 2018

[3]Growth hormone and prolactin in Andrias davidianus: cDNA cloning, tissue distribution and phylogenetic analysis. Yang, Liping,Meng, Zining,Liu, Yun,Zhang, Yong,Liu, Xiaochun,Lu, Danqi,Lin, Haoran,Yang, Liping,Meng, Zining,Liu, Yun,Zhang, Yong,Liu, Xiaochun,Lu, Danqi,Lin, Haoran,Yang, Liping,Huang, Junhai,Lin, Haoran. 2010

[4]Identification and expression of cytochrome P450 genes in the Chinese giant salamander Andrias davidianus. Hu, Qiaomu,Xiao, Hanbing,Tian, Haifeng,Meng, Yan.

[5]Molecular Cloning of Cathelicidin-like cDNA from Andrias davidianus. Yan, M.,Tian, H. F.,Hu, Q. M.,Xiao, H. B.. 2018

[6]Retrospective Survey of Museum Specimens Reveals Historically Widespread Presence of Batrachochytrium dendrobatidis in China. Zhu, Wei,Bai, Changming,Wang, Supen,Li, Xianping,Liu, Xuan,Li, Yiming,Zhu, Wei,Bai, Changming,Wang, Supen,Li, Xianping,Bai, Changming,Soto-Azat, Claudio.

[7]Molecular cloning, characterization and evolutionary analysis of vitellogenin in Chinese giant salamander Andrias davidianus. Tian, Hai-feng,Meng, Yan,Hu, Qiao-mu,Xiao, Han-bing,Hu, Qiao-mu.

[8]Characterization of galectin-1 from Chinese giant salamanders Andrias davidianus and its involvements during immune response. Yang, Hui,Lan, Qingjing,Liu, Ranran,Cui, Dan,Liu, Haixia,Xiong, Dongmei,Liu, Xiaolin,Wang, Lixin,Li, Fenggang.

[9]Identification of Type I IFN in Chinese giant salamander (Andrias davidianus) and the response to an iridovirus infection. Chen, Qian,Chen, Qian,Ma, Jie,Fan, Yuding,Meng, Yan,Xu, Jin,Zhou, Yong,Liu, Wenzhi,Zeng, Xianhui,Zeng, Lingbing.

[10]The effects of dietary betaine supplementation on fatty liver performance, serum parameters, histological changes, methylation status and the mRNA expression level of Spot14 alpha in Landes goose fatty liver. Su, S. Y.,Li, Q. F.,Wang, H. W.,Xie, Z.,Su, S. Y.,Wang, H. W.,Li, X. B.,Li, Q. F.,Dodson, M. V.. 2009

[11]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

[12]Duplicated CCAAT/enhancer-binding protein beta (C/EBP beta) gene: Transcription and methylation changes in response to dietary betaine in Landes goose liver. Yu, S. L.,Li, Q. F.,Xie, Z.,Su, S. Y.,Zhang, X.. 2013

[13]Functional characteristic and differential expression of myostatin in Chlamys nobilis. Zhu, Kecheng,Guo, Huayang,Zhang, Nan,Li, Yundong,Jiang, Shigui,Zhang, Dianchang. 2017

[14]Fasted and postprandial response of serum physiological response, hepatic antioxidant abilities and HSP70 expression in Wuchang bream (Megalobrama amblycephala) fed different dietary carbohydrate levels. Zhou, Chuanpeng,Ge, Xianping,Liu, Bo,Xie, Jun,Chen, Ruli,Zhou, Chuanpeng. 2014

[15]Molecular Cloning and Response to Water Temperature and Nutrient Manipulation of Insulin-like Growth Factor (IGF) Genes in Golden Pompano Trachinotus ovatus (Linnaeus 1758) Larvae. Ma, Zhenhua,Hu, Jing,Liu, Yajuan,Yang, Rui,Sun, Dianrong,Ma, Zhenhua,Sun, Dianrong,Ma, Zhenhua,Qin, Jian G.. 2016

[16]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[17]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[18]Expression profiles of NODs in channel catfish (Ictalurus punctatus) after infection with Edwardsiella tarda, Aeromonas hydrophila, Streptococcus iniae and channel catfish hemorrhage reovirus. Li, Min,Wang, Qi-long,Lu, Yang,Chen, Song-lin,Sha, Zhen-xia,Li, Min,Li, Qi,Lu, Yang. 2012

[19]Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response. Feng, Juan. 2012

[20]Identification and mRNA expression of antioxidant enzyme genes associated with the oxidative stress response in the Wuchang bream (Megalobrama amblycephala Yih) in response to acute nitrite exposure. Sun, Shengming,Ge, Xianping,Zhu, Jian,Xuan, Fujun,Jiang, Xiaojun. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台