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Alteration of cytochrome P450 1 regulation and HSP 70 level in brain of juvenile common carp (Cyprinus carpio) after chronic exposure to tributyltin

文献类型: 外文期刊

作者: Li, Zhi-Hua 1 ; Zhong, Li-Qiao 1 ; Wu, Yan-Hua 1 ; Mu, Wei-Na 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat MOA, Observat Stn Fishery Resource & Environm Upper Mi, Wuhan 430223, Peoples R China

2.Univ South Bohemia Ceske Budejovice, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Fac Fisheries & Protect Waters, Zatisi 728-2, Vodnany 38925, Czech Republic

3.Southwest Univ, Sch Life Sci, Key Lab Aquat Sci Chongqing, Chongqing 400715, Peoples R China

4.Huazhong Agr Univ, Co

关键词: Tributyltin;Fish;EROD activity;Heat-shock proteins;CYP450 1 family genes

期刊名称:FISH PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:2.794; 五年影响因子:2.876 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Tributyltin (TBT), a toxic contaminant in aquatic environments, has bio-accumulated in aquatic food webs throughout the world and can be found at toxic levels in some biota. However, the molecular mechanisms and effects of TBT are not fully understood. The aim of the present study was to investigate the effect of long-term exposure of TBT on cytochrome P450 (CYP450) 1 regulation and heat-shock proteins (HSPs) profiling in brain of freshwater teleost. The effects of long-term exposure to TBT on mRNA expression of cytochrome P450 (CYP450) 1 family genes and ethoxyresorufin O-deethylase (EROD) activity in the brain of common carp were evaluated, as well as HSP 70 level. Fish were exposed to sublethal concentrations of TBT (75 ng/L, 0.75 mu g/L and 7.5 mu g/L) for 15, 30, and 60 days. Based on the results, long-term exposure (more than 15 days) to TBT could lead to obvious physiological-biochemical responses (based on EROD activity, HSP 70 level and CYP450 1 family genes expression). The mRNA expression of CYP450 1 family genes (CYP1A, CYP1B, CYP1C1 and CYP1C2) suggested that CYP1A was to accommodate most EROD activity in fish, but other CYP450 forms also involved in this proceeding. Thus, the measured physiological responses in fish brain could provide useful information to better understand the mechanisms of TBT-induced bio-toxicity and could be used as potential biomarkers for monitoring the TBT pollution in the field.

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