文献类型： 外文期刊

作者： Wen, J. -F. ¹ ; Ruan, G. -L. ¹ ; Zhao, H. -H. ¹ ; Wang, W. -M. ¹ ; Gao, Z. -X. ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

3.Yangtze Univ, Engn Res Ctr Ecol & Agr Use Wetland, Minist Educ, Coll Anim Sci, Jingzhou, Peoples R China

4.Hubei Collaborat Innovat Ctr Freshwater Aquacultu, Wuhan, Hubei, Peoples R China

期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）

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收录情况： SCI

摘要： In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full-length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real-time PCR. Both Kiss and Kissr genes full-length cDNA sequences of M.amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M.amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15hpf stage and lowest in the 30hpf /blastocyst stages for the embryonic period, highest in 7dph/15dph and lowest in 30dph/30dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species.