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Studies of the viral binding proteins of shrimp BP53, a receptor of white spot syndrome virus

文献类型： 外文期刊

作者： Li, Chen 1 ; Gao, Xiao-Xiao 1 ; Huang, Jie 1 ; Liang, Yan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Sustainable Dev Marine Fisheries, Qingdao Natl Lab Marine Sci & Technol, Minist Agr,Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： WSSV;Shrimp;F-1 ATP synthase 13 subunit;BP53;VP37;Interaction

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

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收录情况： SCI

摘要： The specific binding between viral attachment proteins (VAPs) of a virus and its cellular receptors on host cells mediates virus entry into host cells, which triggers subsequent viral infections. Previous studies indicate that F-1 ATP synthase subunit (named BP53), is found on the surface of shrimp cells and involved in white spot syndrome virus (WSSV) infection by functioning as a potential viral receptor. Herein, in a far-western blotting assay, three WSSV proteins with molecular weights of 28 kDa, 37 kDa, and >50 kDa were found to interact with BP53. The 28 kDa and 37 kDa proteins were identified as the envelope protein VP28 and VP37 of WSSV respectively, which could be recognized by the polyclonal antibodies. Enzyme-linked immunosorbent binding assays revealed that VP37 contributed to almost 80% of the binding capability for BP53 compared with the same amount of total WSSV protein. The relationship between BP53 and its complementary interacting protein, VP37, was visualized using a co-localization assay. Bound VP37 on the cell surface co-localized with BP53 and shared a similar subcellular location on the outer surface of shrimp cells. Pearson's correlation coefficients reached to 0.67 +/- 0.05 and the Mander's overlap coefficients reached 0.70 +/- 0.05, which indicated a strong relationship between the localization of BP53 and bound rVP37. This provides evidence for an interaction between BP53 and VP37 obtained at the molecular and cellular levels, supporting the hypothesis that BP53 serves as a receptor for WSSV by binding to VP37. The identification of the viral binding proteins of shrimp BP53 is helpful for better understanding the pathogenic mechanisms of WSSV to infect shrimp at the cellular level. (C) 2016 Elsevier Inc. All rights reserved.

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